HG/T 20719-2021 in English
VALIDTechnical standard for remediation of chemically contaminated soil by microorganism
- Issued on:2021-08-21
- Implemented on:2022-02-01
- File Format:PDF
- Delivery:Via email within 5 business days
$553.00
本文件针对各类化工污染场地中目标污染物微生物降解性好/固化率高的土壤，适用于化工污染土壤微生物修复治理工程（包括修复及风险管控）的方案设计、施工及验收。
主要内容包括总则、术语、基本规定、微生物的筛选及驯化、可行性验证及条件优化实验、微生物放大培养、工程措施、过程监测、安全防护、验收要求等。
Introduction
Standard Formulation Background and Technology Evolution
As the first industry standard for the microbial remediation of chemical-contaminated soil in my country, this specification responds to the requirements of the Soil Pollution Prevention and Control Action Plan and fills the gap in bioremediation technology standards. Compared with the industry status quo of only 10 companies participating in soil remediation around 2010, the current microbial remediation technology has formed a complete technical system including aerobic/anaerobic bacterial flora domestication and composite bacterial agent development.
Core Terminology Analysis
| Terms | Definition Points | Application Cases |
|---|---|---|
| Functional Microorganisms | Strains with the ability to degrade/solidify specific pollutants, including aerobic, facultative anoxic and anaerobic types | The degradation efficiency of Pseudomonas putida in a chemical plant's benzene pollution reached 78% |
| Deep Infiltration Pipe Method | The bacterial solution is infiltrated through a vertical perforated pipe, suitable for permeability coefficients of 6.0×10-5-6.0×10-4 cm/s deep pollution | A pesticide factory used stainless steel seepage pipes (wall thickness 2mm) to repair chlorinated hydrocarbon pollution at a depth of 8m |
Key technical requirements
Nine-step method for microbial screening
- Background analysis: Confirm the type and distribution of pollutants (refer to GB 36600 detection)
- Gradient sampling: Collect 3 groups of representative samples according to the pollutant concentration gradient
- Enrichment culture: Use inorganic salt culture medium + pollutant gradient concentration (sterilization at 121℃ for 20min)
Typical case: A petrochemical site screened out Rhodococcus erythropolis strains, which increased the 30-day degradation rate of petroleum hydrocarbons from the initial 35% to 82%
Key parameters for project implementation
| Parameter type | Remediation project requirements | Risk control requirements |
|---|---|---|
| Organic matter degradation rate | ≥50%/30days | ≥5%/30days |
| Heavy metal solidification rate | ≥80%/30days | ≥30%/30days |
| Bacteria solution dosage | Remediation ≥0.3m³/m³ soil, control ≥0.1m³/m³ soil | |
Special requirements for safety protection
According to GB/T 12801 and HJ/T 415:
- Anti-leakage cofferdams (height ≥ 15cm) are required for the storage of microbial agents
- Heavy metal capture agent emergency kits are required at the heavy metal remediation site
- P3 level protective masks are mandatory at VOCs contaminated sites
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