SC/T 2082-2018 in English
VALIDPyropia haitanensis
- Issued on:2018-12-19
- Implemented on:2019-06-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$156.00
| Standard No: | SC/T 2082-2018 |
| Document status: | VALID |
| Title in English: | Pyropia haitanensis |
| Title in Chinese: | 坛紫菜 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2018-12-19 |
| Implemented on: | 2019-06-01 |
| Professional Classification: | SC-Aquaculture |
| Related Keywords: | pyropia haitanensis
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Introduction
Interpretation of the core content of the standard
This standard establishes a complete technical system for the germplasm identification of Pyropia haitanensis (Pyropia haitanensis). Its innovation is reflected in:
| Detection dimensions | Technical indicators | Key points of the method |
|---|---|---|
| Morphology | Thickness of thallus18-70μm | Visual method + microscope detection |
| Cytogenetics | Chromosome n=5/2n=10 | Improved iron alum-acetic acid magenta staining method |
| Molecular markers | 5.8S(160bp) and rbcS-ISR(516bp) | CTAB method to extract DNA |
Analysis of key technologies
1. Life history characteristics
The standard clearly states that Porphyra haitanensis has typical heterotypic alternation of generations:
• Haploid thallus (gametophyte generation)
• Diploid filament (sporophyte generation)
The spermatotheca division pattern AsBCs and the carpospore division pattern AsB:C are important identification characteristics.
2. Molecular Detection Process
Key steps of DNA extraction:
1) Lyse cells with 65℃ CTAB buffer
2) Deproteinize with chloroform:isoamyl alcohol (24:1)
3) Precipitate DNA with isopropanol
4) Digest RNA contamination with RNase A
Implementation suggestions
- Sampling shall be carried out in accordance with SC/T 2064, and samples shall be ensured to cover different growth stages
- When detecting chromosomes, please note that Carnoy’s fixative must be prepared and used immediately
- For PCR amplification, it is recommended to use the Touch-down procedure to improve specificity
Analysis of Standard Evolution
Compared with the previous version, this standard has the following major upgrades:
• Added rbcS-ISR molecular marker
• Refine the staging standards for filamentous development
• Standardize the sequencing sample preprocessing process
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