SC/T 2090-2020 in English
VALIDSpinyhead croaker
- Issued on:2020-08-26
- Implemented on:2021-01-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$127.00
| Standard No: | SC/T 2090-2020 |
| Document status: | VALID |
| Title in English: | Spinyhead croaker |
| Title in Chinese: | 棘头梅童鱼 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2020-08-26 |
| Implemented on: | 2021-01-01 |
| Professional Classification: | SC-Aquaculture |
| Related Keywords: | spinyhead croaker introduction analysis
body length yellow sea standard technical dimensions sc/t morphological characteristics collichthys lucidus |
| Related Topics: | fish
plum child Acanthus fish in english fish standard Citrus Standard SC/T |
Introduction
Analysis of the core content of the standard
| Technical dimensions | SC/T 2090-2020 | Differences in historical versions |
|---|---|---|
| Morphological indicators | Body length/body height ratio 5.093±0.579, lateral line scales 49-50 | New peritoneum color (white) and number of vertebrae (28-29) |
| Genetic testing | Mitochondrial COI gene fragment 655bp, intraspecific K2P distance <2% | First standardized molecular genetic testing process |
| Reproduction parameters | Absolute egg carrying capacity 3.50×10³-2.23×10⁴ | Clear breeding period in different sea areas (Yellow Sea and Bohai Sea from May to July) |
Key technical points
Morphological characteristics
Collichthys lucidus has an oblong body shape, with the following characteristics: prominent occipital ridges, gray-black front end of gill cavity, and pale yellow fins. Among the measurable traits, the ratio of body length to tail peduncle length is 3.806±0.311, and the head length to eye distance is 4.687±0.522.
Molecular genetics
The standard stipulates that the mitochondrial COI gene detection needs to use primers COLF/COLR, PCR reaction system 50μL, annealing temperature 50℃. Karyotype analysis shows 2n=48t, NF=48, and the chromosome karyotype is shown in standard Figure 2.
Implementation and application suggestions
Testing process optimization
1. Sample collection: According to the requirements of GB/T 18654.2, samples during the breeding period need to be differentiated by sea area
2. Age identification: Otolith annual ring analysis is preferred
3. Molecular testing: It is recommended to add bidirectional sequencing to verify the COI sequence
Standard evolution analysis
This standard integrates morphological and molecular genetic indicators for the first time. Compared with the earlier version:
- Added the anatomical characteristics of 21-22 pairs of swim bladder limbs
- Standardized chromosome karyotype formula expression (2n=48t)
- Appendix A specifies the conditions for DNA extraction and PCR amplification in detail
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