SC/T 7233-2020 in English
VALIDCode of diagnosis for acute hepatopancreatic necrosis disease
- Issued on:2020-08-26
- Implemented on:2021-01-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
| Standard No: | SC/T 7233-2020 |
| Document status: | VALID |
| Title in English: | Code of diagnosis for acute hepatopancreatic necrosis disease |
| Title in Chinese: | 急性肝胰腺坏死病诊断规程 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2020-08-26 |
| Implemented on: | 2021-01-01 |
| Professional Classification: | SC-Aquaculture |
| Related Topics: | acute
necrosis liver diagnostic regulations die pancreas pancreatic streaming necrosis disease diagnosis pancreatic duct Hepatopancreas pancreatic duct pancreatic tissue pancreatic tissue Liver+ liver necrosis pancreas+ Gonad 6 items gonad six around the pancreas miniature pancreas Diagnosis of acute and chronic hepatitis Emergency Eight Rabbit pancreas material Liddell's liver disease Diagnosis of acute cardiac insufficiency Kikuchi disease diagnosis acute insufficiency diagnosis Diagnosis of pancreatic encephalopathy |
Introduction
Analysis of the core content of the standard
| Detection method | Technical points | Judgment standard | Equipment requirements |
|---|---|---|---|
| Histopathological detection | DAFA fixation→paraffin section→HE staining→microscope examination | Epithelial cell shedding + blood cell infiltration | Microtome, microscope |
| Nested PCR | Two rounds of amplification (1269bp/230bp) | Sequencing matching pirA/pirB genes | PCR instrument, electrophoresis system |
| qPCR | TaqMan probe method | Ct≤35 is positive | Fluorescence quantitative PCR instrument |
Key technical implementation points
Sample processing specifications
Hepatopancreatic samples need to be pretreated with DAFA fixative (Appendix A formula) and stored in 70% ethanol after fixation for 24 hours. Molecular detection samples need to be frozen at -20℃ or temporarily stored in 95% ethanol.
Pathological diagnostic features
The typical manifestations of the acute phase are: progressive degeneration of hepatopancreatic tubular epithelial cells → nuclear enlargement → cell rounding and shedding → tubular blood cell infiltration. Attention should be paid to the differential diagnosis from white spot syndrome (WSSV).
Recommendations for the application of the standard
- Three-level diagnostic system: initial screening of clinical symptoms → pathological confirmation → molecular biological quantification
- Quality control: positive/negative/blank controls must be set for each batch of testing
- Cross-validation: PCR-positive samples must undergo GB 4789.7 bacterial species identification
Background of technological evolution
This standard was formulated in response to the outbreak of early mortality syndrome (EMS) of shrimp after 2010. For the first time, it was clearly stated that the pathogen was the Vibrio parahaemolyticus strain (VpAHPND) carrying the pVA1 plasmid. The 2020 version adds a new fluorescent quantitative PCR method, which has a 100-fold increase in detection sensitivity compared to traditional PCR.
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