SF/T 0065-2020 in English

VALID

Determination of 16 tryptamine hallucinogens and their metabolites in hair samples by liquid chromatography- tandem mass spectrometry

Issued on:2020-05-29
Implemented on:2020-05-29
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $270.00
$262.00

Standard No:	SF/T 0065-2020
Document status:	VALID
Title in English:	Determination of 16 tryptamine hallucinogens and their metabolites in hair samples by liquid chromatography- tandem mass spectrometry
Title in Chinese:	毛发中二甲基色胺等16种色胺类新精神活性物质及其代谢物的液相色谱-串联质谱检验方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2020-05-29
Implemented on:	2020-05-29
Chinese Classification:	C06-Legal medical expert
Professional Classification:	SF-
Related Keywords:	liquid chromatography- tandem mass spectrometry introduction analysis liquid nitrogen cryo-grinding technology hair samples mass spectrometry parameter optimization strategy tryptamine hallucinogens
Related Topics:	Two ways to use mass spectrometry Amines Types of Used Mass Spectrometers new used mass spec Types of used liquid phase Liquid Chromatography Requirements MS/MS verification Mass Spectrometry Conditions mass spectrometry Mass spectrometry matrix type Metabolite Detection Secondary Peak Chromatography One Peak One Substance Chromatographic peak time tandem mass spectrometry method Mass Spectrometry Conditions Metabolite Qualification Liquid chromatography isocratic Metabolite Qualification mass spectrometry metabolism Chromatographic peak time mass spectrometry One substance has many liquid chromatographic peaks Chromatographic conditions liquid chromatography tandem mass spectrometry psychoactive substance MS/MS verification Chromatographic conditions liquid chromatography tandem mass spectrometry Liquid Chromatography-Tandem Mass Spectrometry Spectrum material MS 16 Mass Spec Metabolites Mass spectrum amine peak spectral test Amine Gas Chromatography What are the types of chromatographic detectors? Liquid Chromatography of Biogenic Amines in Water Quality Chromatographic peaks of the same substance color active substance Methylbenzylamine liquid chromatography Mass spectrometry+conditions+ Two substances have the same peak in liquid chromatography Mass Spectrometry of Metabolites Chromatography type Chromatographic metabolites Chromatography type Novel Chromatographic Technology Chromatographic polar substance peaks Metabolite Mass Spectrometry Chromatographic conditions: isocratic liquid chromatography Chromatography type Types of liquid chromatography Various types of liquid chromatography Chromatographic peak ethylamine Liquid chromatography-tandem mass spectrometry test method What kind of liquid-solid chromatography Mass spectrometry matrix type Various detectors in liquid chromatography active substance Methylation Detection Mass Spectrometry Liquid Chromatography - Tandem Mass Spectrometry Liquid Chromatography - Tandem Mass Spectrometry Gas Chromatography Amines Full Binary Liquid Chromatography liquid chromatography tandem mass spectrometry Types of Chromatography chromatography substance relative difference Gas Chromatography-Tandem MS/MS active amine mass spec metabolites Mass spectrometry method Types of flight mass spectrometry matrix Liquid Chromatography Tandem liquid chromatography mass spectrometry Detection of Organic Amines in Water by Liquid Chromatography Chromatographic grade tandem mass spectrometry MS/MS What kind of chromatographic classification active amine Cobalamin mass spectrometry detection Liquid Chromatography Amines Mass spectral peak plus 16 Diphenylamine liquid chromatography method and chromatography tandem chromatography Material Chromatography new mass spectrometer Several detectors for liquid chromatography Validation of more than two chromatographic methods Hydroxylamine Liquid Chromatography Liquid Chromatography Secondary Mass Spectrometry Metabolite Chromatographic Peaks Metabolic primary mass spectrometry secondary Distant Spectrum Chromatographic peak time flow cytometry mass spectrometry mass spectrometry metabolism Liquid chromatography and various detectors Second-hand chromatographic mass Colored amines There are many types of chromatography There are many types of chromatography, a liquid chromatography-tandem mass spectrometry base color Mass spectrometry method liquid chromatography tandem mass spectrometry Types of Chromatography Dimethylsulfone liquid chromatography Liquid Chromatography Mass Spectrometry Test Various chromatographic methods are applicable How many polar metabolites are there? polyamine metabolism New drunk driving gas chromatography mass spectrometry inspection agency New column activation Dimethylamine liquid phase detection method Chromatography principles and methods LC/MS method development new psychoactive substances Classification methods of spectra and their differences and connections What are the qualitative methods of chromatography? Mass spectrometry method development How to develop liquid phase of highly polar substances Qualitative methods commonly used in chromatographic analysis and their characteristics Amino acid and metabolite detection methods and principles Liquid Chromatography Accuracy Method development and application of normal phase chromatography systems Four basic types of chromatographic methods Various substances in liquid phase Method for detecting aniline by liquid chromatography Experience in mass spectrometry method development Chiral method development for two-dimensional liquid chromatography Dimethylamine detection chromatographic analysis method t/cpcif0065-2020 sf/t 0030 Amine mass spectrometry method development Detection method of decabromodiphenyl ether and its metabolites

本标准规定了毛发中二甲基色胺等16种色胺类新精神活性物质及其代谢物的液相色谱-串联质谱（LC-MS/MS）检验的试剂、仪器和材料、操作方法以及分析结果评价等要求。
本标准适用于毛发中二甲基色胺等16种色胺类新精神活性物质及其代谢物的定性与定量分析，其它检材中二甲基色胺等16种色胺类新精神活性物质及其代谢物的定性及定量分析可参照使用。

Introduction

Analysis of standard technical framework

Technical elements	Requirements of this standard	Comparison of conventional detection methods
Detection limit	0.01ng/mg	Usually 0.05-0.1ng/mg
Chromatographic column	Acquity UPLC HSS T3(1.8μm)	C18(5μm) is common
Ion source	ESI+Multiple Reaction Monitoring (MRM)	SIM mode was used in some methods

Key operation points

The sample pretreatment used liquid nitrogen cryo-grinding technology, combined with 0.1% formic acid aqueous solution extraction, which significantly improved the recovery rate of hydrophobic tryptamines (83.5-109.7%). The addition of 20mmol/L ammonium acetate buffer system in the mobile phase effectively improved the peak shape of 5-methoxytryptamine derivatives.

Mass spectrometry parameter optimization strategy

In view of the indole parent nucleus characteristics of tryptamines, the standard recommends 5500V ion source voltage and specific collision energy (20-67eV). For example, 5-MeO-DiPT needs to set DP45V/CE20eV, while 4-Acetoxy-DiPT needs DP45V/CE46eV, which reflects the parameter optimization of structural differentiation.

Implementation suggestions

Regularly verify the stability of psilocybin-d4 and psilocybin-d10 internal standards
Pay special attention to the column balance of 4.5-6.5min during the gradient elution phase
It is recommended to add 0.05ng/mg quality control points for each batch of samples

More samples: JJG 21-2008 DL/T 5154-2012 JC/T 2453-2018

Sample only — not a preview of SF/T 0065-2020

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