SN/T 3297-2012 in English

VALID

Detection and identification of Zea mays L.

Issued on:2012-10-23
Implemented on:2013-05-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $310.00
$301.00

Standard No:	SN/T 3297-2012
Document status:	VALID
Title in English:	Detection and identification of Zea mays L.
Title in Chinese:	植物种质资源鉴定方法玉米的鉴定
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2012-10-23
Implemented on:	2013-05-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	morphological identification system identification site key identification method system dual identification path molecular identification key parameters identification
Related Topics:	Phase identification Germplasm resources mbt identification mbt identification sn t 3372 2012 Analysis and identification of substances Germplasm resources Evaluation and Identification of Germplasm Resources Two methods of related substances Identification by MS/MS Identification of Substances in Water sn/t+3438-2012 sn/t+3372-2012 sn+t+3372+2012 Candidate identification method for matrix reference material Secondary identification by mass spectrometry learn from quality analyzer Two methods of protein identification Germplasm identification Germplasm identification sn/t+3367-2012 Identification and Evaluation of Germplasm Resources resource identification Several Identification Methods of Proteins Germplasm Soil identification resource identification sn/t3367-2012 sn/t3209-2012 sn/t3372-2012 in vitro bioassay Several methods of protein identification capital Biometric detector plant identification instrument Secondary identification by mass spectrometry origin of species biological identification method Identification of substances by MS/MS phase identification Identification of water quality corn matter Mass Spectrometric Identification of Substances Several methods of quantification Identification of organic matter plant seed method Phase identification method Phase identification method physical identification Exosome Flow Cytometry Identification Method resource plant origin of species drug identification resource plant plant germplasm plant germplasm meter standard Substance composition identification test Several mass spectrometric identification techniques Quality source Rubber identification Mass Spectrometry for Substance Identification biometric method Basis for Identification of Tree Species sn/t3395-2012 Domestic established mass spectrometric identification Robust mass spectrometric identification Two methods of mass spectrometric identification Substance identification instrument quality analyzer Grain Identification The main method of equipment identification Equipment Identification Method Chemical identification instrument To identify an analytical method unidentified plant Animal identification instrument Seed quality identification Substance analysis and identification method Plant virus identification method Identification of four substances Seed quality identification e identification Verification with multiple applications Methods of Identifying Water Quality Qualification of measuring instruments Identification of moisture Method for Determination of Plant Mass identification equipment Identification of Very Trace Substances Biomarker Identification Methods Identification of corn Phytochemistry \| Western Plant Resources Identification of sheep origin Animal Oil Identification Commonly used methods for structural identification of compounds Material structure identification methods Instrument appraisal qualification Animal breed identification industry Identification of animal mutants Methods for structural identification of compounds Methods for identifying active protein products Hydrocarbon-forming biological identification methods Genetic material identification methods Corn moisture determination method Plant specimen fixation methods Methods for identification of organic impurities in drugs Temperament measurement and identification Rice quality identification method Polymer type identification Rice germplasm resource identification technology Methods for identification of maternal source contamination Fishery germplasm identification Specifications for identification of fishery germplasm Methods for identification of special impurities How to identify strong electrolytes Compound structure identification methods Lipidome identification methods How to measure plant carbon Routine identification methods for iodine preparations Experimental Methods for Identification of Pain Models Methods for identifying compound structures Compound Identification Methods How to identify Lao Wuyuan How to identify compounds Silica resource definition Methods to identify target genes How to establish a mass spectrometry identification method for substances Variety identification industry Common methods for identifying compound structures Methods for identifying new compounds Methods for identifying the structure of compounds National standard method for identification of inorganic substances Cashew nut germplasm resource identification technology How to identify Fushan stone jade Bombay blood group identification method Methods for detecting and identifying substances containing chlorine radicals How to identify amino groups Plant hormone identification method Specific identification methods of virus species Methods for structural identification of peptide impurities Methods to identify protoplast activity How to identify alkaloids What are the methods for identifying the structure of substances? Three methods for identifying stem cells Identification methods of corn varieties

Introduction

Analysis of the core content of the standard

This standard specifies the identification method system for imported corn germplasm resources, integrating equipment technologies such as biological microscopes and PCR amplifiers to establish a dual identification path of morphology and molecular biology.

Morphological identification system

Identification site	Key features	Maize specificity
Root system	Primary root + secondary root + supporting root	Three-level root structure
Stem	Internodes are hollow, aerial roots at the base	Diameter 2-4cm
Male and female inflorescences	Male ears are conical, female ears are axillary	Natural hybridization rate>95%

Comparison of Nine Corn Types

Classification according to GB 553 standard:

Dent type: kernels are sunken like horse teeth, and endosperm is powdery
Hard kernel type: kernels are round and hard, and endosperm is horny
Explosive type: easy to explode when heated, and endosperm is completely horny

Technical Specifications for Molecular Identification

Key Parameters for PCR Detection:

Primer sequence: 5'-GTGCTGCAGGTAGTTGG-3'
Amplification conditions: 94℃/30s→62℃/30s→72℃/30s
Target band: 226bp

Implementation suggestions

1. Morphological preliminary screening is preferred
2. PCR verification is required for difficult samples
3. For identification of wild related species, please refer to Appendix A/B diagrams

More samples: JJG 21-2008 QC/T 942-2021 GB 18306-2015

Sample only — not a preview of SN/T 3297-2012

Loading PDF document...

Error loading PDF. Please make sure the file is valid and try again.

We also recommend

SN 0283-1994 in English

\"Method for Inspection of Dichlorodipyridinol Residues in Exported Poultry Meat\"
1994-02-18
SN/T 0244-1994 in English

\"General Rules for Inspection and Certification of Imported Mechanical and Electrical Instruments\"
1993-08-01
SN/T 4740-2016 in English

Determination of defoliant residues in cotton textile materials for import and export LC-MS/MS method
2016-12-12