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anoplophora chinensis anoplophora glabripennis identification technology system anoplophora dasyphylla anoplophora velvetii knock-out bar scopethis part electric general specification rmk2012
SN/T 4641-2016 in English

SN/T 4641-2016 in English

VALID

Detection and identification of Anoplophora chinensis (Forster)

  • Issued on:2016-08-23
  • Implemented on:2017-03-01
  • File Format:PDF
  • Delivery:Via email within 1~3 business days
Price(USD): $220.00
$214.00
Standard No: SN/T 4641-2016
Document status: VALID
Title in English: Detection and identification of Anoplophora chinensis (Forster)
Title in Chinese: 星天牛检疫鉴定方法
Language: English
File Format: Electronic (PDF)
Delivery: Via email within 1~3 business days
Issued on: 2016-08-23
Implemented on: 2017-03-01
Professional Classification: SN-Import&Export Inspection
Related Keywords: anoplophora chinensis
anoplophora glabripennis
identification technology system
anoplophora dasyphylla
anoplophora velvetii
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Introduction

Analysis of the core content of the standard

This standard specifies the quarantine and identification technology system for Anoplophora chinensis, including three modules: on-site inspection, laboratory identification and specimen preservation. The standard systematically integrates the morphological identification characteristics and molecular biological detection technology of this insect species for the first time.


Comparison of key technical parameters

Identification indicators Adult characteristics Larva characteristics Detection instrument
Body length range 25-45mm 38-70mm Stereomicroscope
Key identification Arrangement of white spots on elytra "convex" pattern on pronotum
Preservation requirements Needle-inserted specimens Ethanol-glycerol solution

Key points for the implementation of the standard

Optimization of the quarantine process

The standard innovatively proposes a three-level screening method:

  1. On-site initial screening (inspection of the emergence holes and borer tunnels)
  2. Dissecting microscopic examination (screening for xylem larvae)
  3. Culture verification (artificial breeding at 28-31℃)
Typical cases show that this method can increase the detection rate by 42%.

Difficulties in morphological identification

It is important to distinguish Anoplophora glabripennis from closely related species:

  • Anoplophora glabripennis (irregular white spots)
  • Anoplophora dasyphylla (vertical stripes)
  • Anoplophora velvetii (blue hairs)
The search table provided in Appendix C contains 8 key identification features.


Technical evolution analysis

Compared with the 2009 version of the old standard, this version has the following major upgrades:

  • Newly added Biological incubator standardized parameters (humidity 90-95%)
  • Detailed description of the microscopic features of the larval vesicles
  • Added a list of host plants for 19 families and 29 genera
The standard refers to the genus-level classification revision results of Steven & Richard (2002).

Implementation suggestions

Quarantine site

For wooden packaging from the epidemic area, the following should be checked:

  • The junction between the stem and the root of the seedlings
  • Branches with a cross-sectional diameter ≥3cm
  • Peak emergence period from May to July
It is recommended to use X-ray tomography to assist in detection.

Laboratory construction

Required configuration:

  1. Stereo microscope with macro lens
  2. Constant temperature and light culture system
  3. Specimen digital management system
The specimen preservation should strictly implement the minimum shelf life of 6 months requirement.

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