SN/T 4730-2016 in English

VALID

Detection and identification of Dickeya chrysanthemi

Issued on:2016-12-12
Implemented on:2017-07-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $260.00
$253.00

Standard No:	SN/T 4730-2016
Document status:	VALID
Title in English:	Detection and identification of Dickeya chrysanthemi
Title in Chinese:	菊基腐病菌检疫鉴定方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2016-12-12
Implemented on:	2017-07-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	dickeya chrysanthemi dickeya chrysanthemi introduction standard overview main identification characteristics double identification process similarity ≥0.75 key identification characteristics
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Introduction

Standard Overview and Technical Background

SN/T 4730-2016 is a plant quarantine industry standard issued by the General Administration of Customs of China. It provides standardized methods for the detection and identification of Xanthomonas chrysanthemi (Dickeya chrysanthemi). This pathogen can cause soft rot in over 200 plant species, including chrysanthemums and potatoes, and is of significant international quarantine importance.

Core detection methods

Method type

Culture medium

Culture conditions

Judgment index

Isolation and culture

Nutrient agar (NA)

28℃/2-4 days

Milky white colonies, diameter 0.5-1mm

Biochemical identification

Thornley medium

24℃/3-7 days

Oxidase negative, pectinase positive

Molecular identification

Biolog

GenⅢ

30℃/18h

Similarity ≥0.75

Key identification characteristics

This bacterium belongs to the class γ-Proteobacteria. The main identification characteristics include:

Gram-negative short rods (0.5-0.7μm×1.0-2.5μm)
Shows a typical filamentous reaction in KOH solution
Cannot utilize citrate and malonate
Sensitive to erythromycin (10μg)

Comparison of technical evolution

Taxonomic changes	1984	2005	2012
Generic name	Erwinia	Dickeya	Dickeya
Biological variants	6	2(bv5/bv6)	Integrated into subspecies
Molecular markers	Physiology and biochemistry	16S rRNA	DNA-DNA hybridization

Implementation Recommendations

Suspected samples should be stored at -80℃ for no more than 3 months
A double identification process is recommended: Biolog system initial screening + PCR confirmation
Pay attention to identification with similar bacterial species (Pectobacterium)
For quarantine treatment, it is recommended to soak in 70℃ hot water for 10 minutes

More samples: JGJ 94-2008 QC/T 942-2021 JC/T 2453-2018

Sample only — not a preview of SN/T 4730-2016

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