SN/T 4748-2017 in English

VALID

Quarantine protocol for lymphocytic choriomeningitis

Issued on:2017-05-12
Implemented on:2017-12-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $200.00
$194.00

Standard No:	SN/T 4748-2017
Document status:	VALID
Title in English:	Quarantine protocol for lymphocytic choriomeningitis
Title in Chinese:	淋巴细胞性脉络丛脑膜炎检疫技术规范
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-05-12
Implemented on:	2017-12-01
Professional Classification:	SN-Import&Export Inspection
Related Topics:	Lymphocytes alpha cells About Lymphocytes Cell membrane analyzer single cell technology Lymphocytes eppendorf lymphocyte wall Venous mononuclear cells Venous mononuclear cells if cell cell membrane mural lymphocytes b cell inflammation network cell cell network Lymphocytes single cell technology primary lymphocyte Cell Transient Technology Lymphocyte immunoassay upstream primer meningitis atypical lymphocyte Cellular cellular lymphoma Lymphocyte immunoassay Lymphocyte manipulation Many lymphocytes mucosal cells upstream primer chronic lymphatic Cellular Choroid plexus Naomaining network cell context Lymphadenitis T lymphocyte proliferation Major Engineering of Lymphocytes Cell Immunoassay lymphocyte immunity mantle cell lymphoma Cellular immunoassay method corneal cells cell membrane method Inflammatory Cell Section How Lymphocytes Proliferate immune lymphocyte Six items of lymphocyte immune analysis technical cell membrane encephalopathy cell if Lymphocyte monoclonal cell membrane technology "meningitis Lymphocyte blastification Lymphocyte immunity 2 items primary lymphocyte Lymphocyte immunity 4 items cell membrane structure lymphocyte markers T lymphocyte analyzer Lymphocytes per cubic millimeter Lymphocyte Transformation Assay Proliferation of T lymphocytes Technology detection cells unconventional lymphocytes lymphocyte target target lymphocyte from the cell membrane Lymphocyte circulation process immunity Lymphocyte immortalization technology Lymphocyte separation solution principle free lymphocyte-like cells Principles of Lymphocyte Separation Cerebrospinal fluid cell classification lymphoid micromegakaryocytes Cell membrane extraction method Cell membrane disruption method Cell membrane integrity detection method Cell membrane phospholipid detection method Evaluation of cell membrane permeability membrane cytotoxicity Inflammatory cell detection method lymphocyte counting method Small lymphocytic lymphoma evaluation Cell membrane fusion method Cell membrane integrity detection method Cell membrane permeability detection method sn/t 4953-2017 How to break cell membrane sn/t 4972-2017 sn/t 4921-2017

Introduction

Analysis of Standard Technical Framework

Detection Method	Sensitivity	Specificity	Applicable Scenarios
Fluorescent RT-PCR	10^3 copies/μL	100%	Early Pathogen Detection
ELISA	1:50 dilution	98.7%	Antibody Screening

Key Technical Points

1. Fluorescent RT-PCR Detection System

Degenerate primers (LCMV-F/R) and a TaqMan probe (LCMV-P) were designed targeting the LCMV NP gene fragment. Their unique polynucleotide design (including degenerate bases such as D/K/R/N) covers 99.6% of known viral strain variations.

2. ELISA Antigen Preparation

The standard specifies two antigen sources: Vero cell culture antigens must be concentrated by ultracentrifugation, while genetically engineered antigens must have their epitope integrity verified. The threshold for positive detection requires an OD value ≥ 2 times that of the negative control.

Implementation Difficulties and Solutions

Biosafety Control

Pathogen detection must be carried out in a BSL-2 laboratory. Attention should be paid to sample processing:
① Tissue grinding must be completed in a biosafety cabinet
② Waste materials must be autoclaved at 121℃ for 30 minutes

Nucleic Acid Extraction Quality Control

The standard clearly requires:
① Positive control Ct value ≤30
② Suspicious samples (35 ③ The number of freeze-thaw cycles should not exceed 3 times

Background of Standard Evolution

Main updates of the 2017 version:
① Adding hamsters as applicable animals
② Introducing degenerate primer technology to improve strain coverage
③ Standardize the equivalence verification requirements for commercial test kits

Typical application case

An outbreak of cases of unexplained neurological symptoms occurred in an experimental animal center. Using this standard, the following tests were performed:
① Fluorescent RT-PCR detected a Ct value of 28.3 in brain tissue
② ELISA showed a serum OD value of 0.892 (negative control 0.152)
The diagnosis was confirmed to be LCMV infection, and tracing back to the source revealed that the virus was introduced into wild mice.

More samples: DL/T 5154-2012 QC/T 942-2021 NB/T 47013.2-2015

Sample only — not a preview of SN/T 4748-2017

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