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identification requirements stereomicroscope identification ambl yomma com pressum special enamel pattern typical identification case core identification features locationfemale characteristicsmale characteristics coalbed methane wells hormone-related secondary metabolites indicator plant method introduction technical background intelligent medical decision-making
SN/T 5102-2019 in English

SN/T 5102-2019 in English

VALID

Identification of Ambl yomma com pressum (Macalister, 1872)

  • Issued on:2019-09-03
  • Implemented on:2020-03-01
  • File Format:PDF
  • Delivery:Via email within 1~3 business days
Price(USD): $130.00
$127.00
Standard No: SN/T 5102-2019
Document status: VALID
Title in English: Identification of Ambl yomma com pressum (Macalister, 1872)
Title in Chinese: 侧扁花蜱鉴定方法
Language: English
File Format: Electronic (PDF)
Delivery: Via email within 1~3 business days
Issued on: 2019-09-03
Implemented on: 2020-03-01
Professional Classification: SN-Import&Export Inspection
Related Keywords: identification requirements
stereomicroscope identification
ambl yomma com pressum
special enamel pattern typical identification case
core identification features locationfemale characteristicsmale characteristics
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Introduction

Technical background of the standard

This standard is formulated to meet the quarantine and identification requirements of Amblyomma compressum Macalister, 1872, an important quarantine pest belonging to the family Ixodidae of the order Ixodida. This species has been historically known by three synonyms, including Haemalastor compressum.


Core identification features

LocationFemale characteristicsMale characteristics
Pseudo-head base 6.8mm wide, obvious lateral angles 5.0-5.2mm wide, blunt lateral angles
Pedals Second segment has a long dorsal spur Second segment has a dentate process on the ventral side
Shield Oval, with little enamel Fully covered, with special enamel pattern

Typical identification case

In 2018, a suspected individual was detected from the surface of imported monitor lizards at Shenzhen Port. Based on the female anal groove characteristics described in Article 7.3.1 of this standard (circumferential and U-shaped at the front) and the key indicator of valve plate comma shape, it was finally confirmed as the first interception at a Chinese port.


Key points of technical implementation

  1. Sample processing: Use 75% ethanol to fix, and keep the specimen horizontal when measuring under a stereomicroscope
  2. Identification of closely related species: Focus on comparing the scutellum pattern and basal gland structure of the Javan Ornithodoros tick (A. javanense)
  3. Molecular verification: It is recommended to sequence and align the COI gene, and the sequence similarity should be ≥98%

Analysis of standard evolution

Compared with the 2009 version of the GB/T 1.1 basic standard, this standard establishes a complete morphological indicator system for Compressed Ornithodoros tick for the first time:

  • Added 12 microscopic measurement data (such as the length-to-width ratio of the second segment of the female pedipalp is 1.7:1)
  • The threshold for determining the subcircular structure of the valve plate is clarified
  • The requirements for the extension angle of the foot IV during specimen preparation are standardized

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