SN/T 5122-2019 in English
VALIDDetermination of quinolones in live animal and feeds for import and export - Colloidal gold immun eassay
- Issued on:2019-09-03
- Implemented on:2020-03-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$97.00
Introduction
Technical background of the standard
This standard is formulated to determine the residual risk of nine quinolone drugs, including enrofloxacin and ciprofloxacin, in imported and exported edible animals and feeds. It uses colloidal gold immunochromatography technology to achieve rapid screening, with a detection limit of 1-10μg/kg.
Key Detection Process
| Sample Type | Pretreatment Method | Detection Time | Sensitivity |
|---|---|---|---|
| Animal Tissue | PBS Extraction + Centrifugation | 20min | 10μg/kg |
| Liquid Feed | Direct Dilution Method | 15min | 50μg/kg |
| Solid Feed | Acidified Acetonitrile Extraction | 30min | 300μg/kg |
Technical Points Analysis
1. Sample Pretreatment
Tissue samples need to be homogenized with 1% formic acid aqueous solution and then centrifuged. Feed samples are purified using solid phase extraction columns. Key control points include:
- pH value adjusted to 8.0±0.5
- Centrifugal speed ≥10000r/min
- Filtered using 0.22μm filter membrane
2. Colloidal gold test strip judgment
The result interpretation needs to be carried out at 15-25℃, and the color intensity of the control line (C line) and the test line (T line) is compared:
- Positive: T line has no color or is weaker than C line
- Negative: T line is equal to/deeper than C line
Implementation recommendations
- Standard solution should be stored at 4℃ away from light, with a validity period of 12 months
- Different sample matrices should be validated, and the recovery rate should be ≥80%
- It is recommended to conduct a positive control test for each batch of testing
Technology evolution analysis
Compared with the traditional HPLC method, the colloidal gold method in this standard shortens the detection time from 4 hours to 30 minutes, which is more suitable for rapid screening at ports. However, it should be noted that this method is a preliminary screening method, and positive results need to be confirmed using standards such as GB/T21312.
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