SN/T 5137-2019 in English
VALIDDetection and identification of Monacha cartusiana (Müller, 1774)
- Issued on:2019-09-03
- Implemented on:2020-03-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$146.00
| Standard No: | SN/T 5137-2019 |
| Document status: | VALID |
| Title in English: | Detection and identification of Monacha cartusiana (Müller, 1774) |
| Title in Chinese: | 华僧螺检疫鉴定方法 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2019-09-03 |
| Implemented on: | 2020-03-01 |
| ICS Classification: | 65.020.01-Farming and forestry in general |
| Chinese Classification: | B16-Plant quarantine, pest control |
| Professional Classification: | SN-Import&Export Inspection |
| Related Topics: | jpk
Phase identification method Phase identification method Inspection and Quarantine Identification Fenghua Immunity ISKNV quarantine Warfarin detection method |
Introduction
Analysis of the standard technical framework
| Detection dimensions | Traditional methods | This standard method | Technical innovations |
|---|---|---|---|
| Morphological identification | External feature observation only | Comprehensive identification of shell + reproductive system | Add microscopic feature analysis of radula |
| Molecular detection | No standard process | COI gene sequence analysis | Set ≥95% sequence similarity threshold |
Core detection elements
Key morphological features
The standard stipulates that the identification characteristics of Monacha cartusiana include:
- Shell size: height 6-10mm, width 9-18mm
- Reproductive system characteristics: Penile with whip-like body, copulatory sac pore is V-shaped
- Ring tongue structure: central teeth are single-pointed, lateral teeth are bi-pointed
Molecular detection process
The standard uses the COI gene fragment (658bp) for species confirmation:
- DNA extraction: extracted from foot muscle tissue using the SDS method
- PCR amplification: primers LCO1490/HCO2198
- Sequence alignment: with GenBank KM247389 reference sequence alignment
Implementation recommendations
Key points for quarantine site operations
1. Sample preservation: Living samples must be fixed with 75% alcohol for at least 24 hours
2. Morphological identification: A stereo microscope (40-80 times)
3. Molecular detection: It is recommended to set up a positive control (KM247389)
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