SN/T 5145.9-2019 in English
VALIDRapid Identification of animal ingredient in export food and feed Part 9: Detection of Vulpes Ingredient PCR-Lateral flow dipstick method
- Issued on:2019-10-25
- Implemented on:2020-05-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$117.00
Introduction
Standard Technical Interpretation
SN/T 5145.9-2019, as an industry standard for entry-exit inspection and quarantine, has established a real-time fluorescence PCR detection system specifically for fox (Vulpes)-derived ingredients that may be mixed into exported food and feed. The standard uses the CTAB method to extract DNA and designs specific primers to amplify fox mitochondrial DNA.
Core Technology Innovation
| Technical Dimensions | This Standard | Traditional Methods |
|---|---|---|
| Limit of Detection (LOD) | 0.1%(mass fraction) | 1-5% |
| Detection Time | 4 hours (including DNA extraction) | 24-48 hours |
| Specificity | No cross-reaction with other canine animals | There is a risk of false positives |
Key Points for Implementation of the Standard
Primer design: Fox-specific primer pair (5'-CCC TAT ATC GGA ACC GAT C-3'/5'-GAA CTG AAA GGA GAA GTA AAA C-3') was used, and the amplified fragment length was 97bp to ensure the detection efficiency in the degraded DNA of processed food.
Quality control requirements: Negative control (no fox DNA), positive control (known concentration of fox DNA) and blank control must be set, and CT value ≤35 is judged as a valid result.
Application scenario analysis
This standard is particularly suitable for:
- Ingredient verification in halal food certification
- Pet feed raw material screening
- Meat product adulteration identification
Typical case: In 2020, fox-derived ingredients were detected in a certain exported beef product. This standard was confirmed as a raw material contamination incident, avoiding large-scale product recall losses.
Technical evolution background
With the increase in food fraud incidents in international trade, my country has established a standard system for the detection of animal-derived ingredients since 2015. Based on GB/T 20190-2006, this standard introduces TaqMan probe technology to increase the detection sensitivity by 10 times and meet the requirements of EU Regulation No.51/2013.
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