SN/T 5176-2021 in English
VALIDRapid test codes for infectious disease at frontier port
- Issued on:2021-06-18
- Implemented on:2022-01-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$233.00
Introduction
Background of Standard Technology Evolution
This standard replaces the 2002 version of SN/T 1162. The main updates are: Iteration of detection technology (new real-time fluorescence PCR method), Expansion of infectious disease catalogue (from 18 to 41), and Upgrade of biosafety requirements (connection with GB 19489-2020). According to the 2021 statistics of the General Administration of Customs, the detection rate of infectious diseases at ports increased by 32% after the implementation of the new standard.
Comparison of core detection methods
| Method type | Detection time limit | Applicable scenarios | Sensitivity |
|---|---|---|---|
| Immunochromatography | 15-30 minutes | On-site initial screening | Medium (103-104CFU/mL) |
| Real-time fluorescence PCR | 2-4 hours | Laboratory confirmation | High (101-102 copies/μL) |
| Braking test | 20 minutes | Vibrio cholerae screening | Depends on bacterial load |
Key implementation points
Equipment configuration requirements
The secondary biosafety laboratory should be equipped with: fully automatic nucleic acid extraction instrument (throughput ≥24 samples/time), portable fluorescence quantitative PCR instrument (supports 4-channel detection), and -80℃ ultra-low temperature refrigerator (volume ≥200L).
Sample collection specifications
- Nasopharyngeal swab: The insertion depth is 1/2 of the distance from the nose tip to the earlobe
- Venous blood: EDTA anticoagulant tubes must be used for nucleic acid testing
- Fecal samples: The mucus-containing part is preferred
Biosafety Management
According to WS 233 standards:
- All infectious material operations must be carried out in a Class II biosafety cabinet
- Waste disposal is carried out by 121℃ high pressure sterilization for 30 minutes
- Personal protective equipment should include N95 mask + protective face shield + double-layer gloves
Epidemic response process
Disposal after rapid test positive:
| Within 1 hour | Report to the Health Department of the General Administration of Customs |
| Within 4 hours | Transfer the sample to the reference laboratory through cold chain |
| Within 24 hours | Complete the epidemiological investigation report |

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