SN/T 5185-2020 in English
VALIDQuarantine protocol for swine paratyphoid
- Issued on:2020-12-30
- Implemented on:2021-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$418.00
| Standard No: | SN/T 5185-2020 |
| Document status: | VALID |
| Title in English: | Quarantine protocol for swine paratyphoid |
| Title in Chinese: | 猪副伤寒检疫技术规范 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2020-12-30 |
| Implemented on: | 2021-07-01 |
| Professional Classification: | SN-Import&Export Inspection |
| Related Topics: | typhoid fever
astm5185 astm 5185 paratyphoid Quarantine technology ISKNV quarantine |
Introduction
Analysis of the core content of the standard
This specification establishes a complete technical system for swine paratyphoid quarantine, including requirements for key equipment such as incubators and PCR instruments, and specifies the entire process from sample collection to result determination.
Comparison of detection methods
| Method | Sensitivity | Time consumption | Applicable scenarios |
|---|---|---|---|
| Bacterial isolation | Medium(10^3 CFU/g) | 3-5 days | Pathogen confirmation |
| ELISA | High(Antibody detection) | 6 hours | Large-scale screening |
| Real-time fluorescence PCR | Extremely high(10 copies/μL) | 2 hours | Rapid diagnosis |
Technical points
Characteristics of typical cases
Acute type: body temperature 41-42℃ with skin purpura, pathologically showing rubbery spleen; Chronic type: obvious features of necrotizing enteritis, cecal mucosa with bran-like ulcers.
Key biochemical identification
Salmonella appears as red on the bevel (K), yellow on the bottom (A) in triple sugar iron agar, produces H2S, and is positive for lysine decarboxylase, which needs to be confirmed by serology (O/H antigen).
Recommendations for the implementation of the standard
- Laboratories should establish biosafety cabinet operation specifications in accordance with GB 19489 requirements
- PCR and bacterial isolation are recommended to be used together, with a positive compliance rate of 98.7%
- ELISA testing is recommended to be considered suspicious when P/N ≥ 1.5, and epidemiological investigations should be combined
Technology evolution background
This standard incorporates the real-time fluorescence PCR method for the first time, which shortens the detection cycle from 72 hours to 4 hours compared to the traditional culture method, and introduces CHROMagar chromogenic culture medium to improve separation efficiency.
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