SN/T 5192-2020 in English
VALIDQuarantine protocol for dermatophilosis
- Issued on:2020-12-30
- Implemented on:2021-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$262.00
| Standard No: | SN/T 5192-2020 |
| Document status: | VALID |
| Title in English: | Quarantine protocol for dermatophilosis |
| Title in Chinese: | 嗜皮菌病检疫技术规范 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2020-12-30 |
| Implemented on: | 2021-07-01 |
| Professional Classification: | SN-Import&Export Inspection |
| Related Keywords: | quarantine protocol
inhibit miscellaneous bacteria improve isolation success rate molecular detection dermatophilosis introduction analysis new pcr primer sequences detection sensitivity edition improvement points pathogen isolation basic culture medium add polymyxin b |
| Related Topics: | superbugs
Skin Leather + Leprosy mycosis addiction Scab Quarantine technology Scleroderma canca pr3 Oral Thermophilus Subcutaneous pathogen testing ISKNV quarantine White tail disease quarantine technology Inspection and quarantine of quarantine pests and diseases Border disease quarantine technology |
Introduction
Analysis of the Standard Technical Framework
| Technical Module | 2002 Edition | 2020 Edition | Improvement Points |
|---|---|---|---|
| Pathogen Isolation | Basic Culture Medium | Add Polymyxin B to Inhibit Miscellaneous Bacteria | Improve Isolation Success Rate |
| Molecular Detection | Not Included | New PCR Primer Sequences | Detection Sensitivity Increased by 103 Times |
| Serological Test | Fluorescent antibody only | ELISA determination formula added | Quantitative detection is more accurate |
Details of core detection technology
1. Key steps in pathogen isolation
The standard requires the use of a tissue grinder to prepare a 10% diseased material suspension, which is then cultured at 37°C for 4-7 days on sheep blood agar medium containing polymyxin B. The characteristic colonies are as follows:
- 24-48h: yellow-gray rough colonies (1mm in diameter)
- After 72h: light yellow smooth colonies appear
2. Innovation in molecular biology detection
Newly added specific primer pairs:
FP: 5'-ACATGCAAGTCGAACGATGA-3' RP: 5'-ACGCTCGCACCCTACGTATT-3'
Amplification conditions: 94℃ 30s → 60℃ 30s → 72℃ 60s, 35 cycles, expected product 500bp.
Implementation suggestions
Special tips for biosafety
According to the requirements of GB 19489, during operation, you need to:
- Wear a protective mask and goggles
- Use 0.1% mercuric chloride water to disinfect instruments
- Speed specimens should be handled in a Class II biosafety cabinet
Technology evolution analysis
Main breakthroughs of this standard:
- Establish a multi-technology combination judgment system (clinical + separation + PCR + serology)
- Introduce the S/P value calculation formula to quantify ELISA results
- Standardize the entire process of molecular diagnosis from DNA extraction to sequence determination
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