SN/T 5193-2020 in English
VALIDQuarantine protocol for rabbit coccidiosis
- Issued on:2020-12-30
- Implemented on:2021-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$262.00
| Standard No: | SN/T 5193-2020 |
| Document status: | VALID |
| Title in English: | Quarantine protocol for rabbit coccidiosis |
| Title in Chinese: | 兔球虫病检疫技术规范 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2020-12-30 |
| Implemented on: | 2021-07-01 |
| Professional Classification: | SN-Import&Export Inspection |
| Related Topics: | rabbit
Rabbit Quarantine technology Quarantine Pupa GB/T 5193-2020 Inspection and quarantine of quarantine pests and diseases Border disease quarantine technology Rabbit specification query Standardized Rabbit |
Introduction
Analysis of standard technical framework
| Technical modules | Core requirements | Innovation points |
|---|---|---|
| Sample collection | Dual-period sampling of feces of live rabbits (1st/12th day of isolation) | Establishment of a dynamic monitoring mechanism |
| Morphological identification | Sporeization culture of oocysts (28℃ 48-72h) | Standardized potassium dichromate solution treatment |
| PCR detection | ITS1 universal primers (annealing at 58℃) | Simultaneous detection of multiple worm species |
Detailed explanation of key detection technologies
1. Morphological identification system
The standard stipulates that an optical microscope (400×) should be used to observe the characteristics of the oocysts of 11 species of Eimeria, with key identifications:
- Eimeria stuartii: the only worm species that parasitizes the bile duct (average 33.6μm×21.3μm)
- Large Eimeria: dike-like microporous structure (35.0μm×24.0μm)
- Eimeria perforata: the smallest oocyst (22.7μm×14.2μm)
2. Molecular detection scheme
The PCR reaction system used a 20μL final volume, and the key parameters were:
Cycling conditions: 94℃ 2min → (94℃ 1min → 58℃ 1min → 72℃ 1min)×32 → 72℃ 7min
Sequencing judgment required comparison with the GenBank database for ≥97% homology
Recommendations for implementation of the standard
Application case in farms
In a mixed infection scenario in a rabbit farm, after separating oocysts using a refrigerated centrifuge:
- Morphological initial screening revealed a pear-shaped/oval mixed oocyst group
- PCR amplification obtained a 489bp band
- Sequencing comparison confirmed infection with Eimeria intestinalis (E. intestinalis)
Technology Evolution Analysis
Compared with the traditional saturated salt water flotation method, this standard:
- Introduced potassium dichromate sporulation culture (2.5% solution) to improve the activity of oocysts
- Used ITS1 conserved region primers to solve the cross-reaction problem
- Specified sequencing verification as the gold standard for diagnosis
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