SN/T 5228.8-2019 in English
VALIDRapid detection of pathogens in export food-MALDI-TOF MS method Part 8: Klebsiella pneumoniae
- Issued on:2019-12-27
- Implemented on:2020-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$117.00
| Standard No: | SN/T 5228.8-2019 |
| Document status: | VALID |
| Title in English: | Rapid detection of pathogens in export food-MALDI-TOF MS method Part 8: Klebsiella pneumoniae |
| Title in Chinese: | 出口食品中病原微生物快速筛选方法 MALDI-TOF MS法 第8部分：肺炎克雷伯氏菌 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2019-12-27 |
| Implemented on: | 2020-07-01 |
| Professional Classification: | SN-Import&Export Inspection |
| Related Keywords: | rapid detection
klebsiella pneumoniae microbial detection detection range 48-72-hour detection cycle |
| Related Topics: | quick filter
Microbial Mass Spectrometry maldi-tof-ms method Rapid detection of pathogenic microorganisms tof ms ms method Microbial Screening Methods method tof Klebsiella Klebsiella rapid screening method maldi tof method ms method+ tof/ms microbial ms maldi/tof method+tof rapid pneumonia maldi-tof-ms method sn/t 2019 MALDI-TOF MS Klebsiella pneumoniae Watson Biopneumonia maldi-tof Methods for screening clones |
Introduction
Technical Background and Significance of the Standard
As an innovative application of MALDI-TOF MS technology in the field of food microbial detection, this standard establishes a 20-minute rapid screening scheme for Klebsiella pneumoniae. Compared with the 48-72-hour detection cycle of the traditional culture method, mass spectrometry technology improves efficiency by more than 90% while maintaining >95% identification accuracy.
Analysis of core detection process
| Steps | Key parameters | Technical points |
|---|---|---|
| Sample pretreatment | BPW enrichment 18-24h | Homogenization speed 8000-10000r/min |
| Isolation and culture | Cultivation on DHL plate at 36℃ | Characteristic mucous colonies |
| Mass spectrometry detection | Laser frequency 60Hz | Detection range of 2000-20000Da |
Key parameters of mass spectrometry technology
Experimental data show that when α-cyano-4-hydroxycinnamic acid (CHCA) is used as the matrix, the best signal-to-noise ratio can be obtained at an accelerating voltage of 20kV. Typical characteristic peaks include:
- Ribosomal protein S10 (m/z 5250)
- DNA binding protein (m/z 10300)
Implementation suggestions and precautions
1. Quality control: BTS standards must be used for calibration before each test, and the mass-to-charge ratio error must be <0.03%
2. Biosafety: All cultures must be sterilized at 121℃ for 30 minutes
3. Result verification: Matching scores ≥2.3 can be reported directly, and 1.7-2.2 must be verified in combination with GB/T 14926.13
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