SN/T 5287-2021 in English
VALIDTextiles for import and export - Determination of bisphenol A (BPA) - Surface plasmon resonance (SPR) method
- Issued on:2021-11-22
- Implemented on:2022-06-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$175.00
Introduction
Technical Background and Evolution of Standards
With the strengthening of global control over chemical residues in textiles, bisphenol A (BPA) has been listed as a key monitored substance as a typical environmental hormone. Traditional detection methods such as HPLC have problems such as complex pre-treatment and high cost. This standard applies surface plasmon resonance to textile BPA detection for the first time, achieving a high-sensitivity determination of 0.02 mg/kg.
Analysis of core principles
| Technical elements | Traditional HPLC method | SPR method |
|---|---|---|
| Detection mechanism | Chromatographic separation + UV detection | Antigen-antibody specific binding |
| Sample pretreatment | Complex purification steps | Methanol ultrasonic extraction |
| Detection limit | 0.05 mg/kg | 0.02 mg/kg |
| Single sample detection time | ≥30 minutes | ≤20 minutes |
Key Operation Specifications
1. Chip Modification Process
The use of CM5 chip must strictly follow the three-step modification method of activation (EDC/NHS mixture) → coupling (BPA-BSA antigen) → blocking (BSA solution), and the flow rate is controlled at 10 μL/min.
2. Standard Curve Establishment
The BPA concentration gradient is required to be 10n μg/L (n=0-10), and the four-parameter Logistic curve fitting is used, and the correlation coefficient R2 should be ≥0.99.
Implementation Difficulties and Solutions
Case: In a laboratory, the signal attenuation after chip regeneration was more than 20%. After investigation, it was found that the glycine-hydrochloric acid solution (pH3.6) was not replaced in time. It is recommended to update the regeneration solution every 50 tests or every week.
Method Validation Data
| Validation Items | Requirements | Measured Data |
|---|---|---|
| Precision RSD | ≤25% | 18.7% |
| Spike Recovery | 80-120% | 92.3-105.6% |
| Chip Service Life | Signal Attenuation≤20% | Average 35 times |
Industry Application Recommendations
1. It is recommended to include BPA in the routine testing items for textiles exported to the EU;
2. When selecting an SPR instrument, verify its temperature control accuracy (±0.1°C);
3. Establish a daily calibration system for chip performance and record baseline drift values.
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