SN/T 5382-2021 in English
VALIDldentification of Cibotium barometz (L.) J. Sm.
- Issued on:2021-06-18
- Implemented on:2022-01-01
- File Format:PDF
- Delivery:Via email within 5 business days
$359.00
Introduction
Analysis of the core content of the standard
This document establishes a standardized identification system for Cibotium barometz, including:
- Morphological identification: 7 major characteristics such as golden hairs on the rhizome and tripinnate leaves
- Molecular biology-assisted identification: Sequencing of a 528bp fragment of the psbA-trnH gene
- Identification of similar species: 4 key differences from Cibotium barometz
Comparison of key technologies
| Identification dimensions | Cibotium barometz | Cibotium barometz of the Philippines |
|---|---|---|
| Distribution of pinnules | Present on both sides of the pinna | Absent on the lower side of the base |
| Lobe morphology | Sickle-shaped | Lanceolate |
| Number of sori | 1-5 pairs | 1-3 pairs |
| Spore characteristics | With equatorial ring | Without equatorial ring |
Key points for implementation of the standard
Morphological identification process
- Observe the golden cushion-like hairs on the rhizome (confirmed by stereomicroscope)
- Inspect the "Ruyi cloud-shaped" cross section of the petiole vascular bundle
- Measure the size of the spore sori (115-165×70-110μm)
- Observe the rib-like patterns of the spores under a scanning electron microscope
Precautions for molecular detection
- Liquid nitrogen pretreatment is required when extracting DNA by CTAB method
- PCR amplification annealing temperature is strictly controlled at 56℃
- Sequence alignment requires GenBank similarity ≥98%
Background of technical evolution
This standard combines scanning electron microscopy spore observation (Appendix C) with DNA barcoding technology (Appendix E) for the first time, solving the problem that traditional taxonomy has difficulty in identifying fragmented samples. The adjustment of the classification system of the genus Corymbopteris in the 2013 English revised edition of Flora of China provides a theoretical basis for this standard.
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