SN/T 5462-2022 in English
VALIDIdentification of Dendrobium nobile Lindl.
- Issued on:2022-07-07
- Implemented on:2023-02-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$262.00
| Standard No: | SN/T 5462-2022 |
| Document status: | VALID |
| Title in English: | Identification of Dendrobium nobile Lindl. |
| Title in Chinese: | 金钗石斛鉴定方法 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2022-07-07 |
| Implemented on: | 2023-02-01 |
| Professional Classification: | SN-Import&Export Inspection |
| Related Keywords: | morphological identification characteristic parts identification points
morphological identification traditional morphological identification dna barcode identification legal identification basis |
| Related Topics: | sn/t2022
diamond identification method Southern Dendrobium diamond identification method Phase identification method Phase identification method Exosome Flow Cytometry Identification Method Dendrobium alkaline detection method Hydrocarbon-forming biological identification methods Dendrobium wine How to identify strong electrolytes sn/t 5380-2022 How to identify californium metal |
Introduction
Analysis of the core content of the standard
This standard specifies a dual method system of morphological identification and DNA barcode identification for Dendrobium nobile Lindl., which is applicable to the import and export inspection and quarantine of Chinese medicinal materials.
Key features of morphological identification
| Characteristic parts | Identification points | Typical parameters |
|---|---|---|
| Stem | Upright fleshy cylindrical, internodes inverted conical | 10-60cm long, up to 1.3cm thick |
| Leaf | Oblong, with a sheath at the base | 6-11cm long, 1-3cm wide |
| Flower | Labium broad ovate densely covered with short hairs | With purple-red patches and eyelashes on the edges |
Molecular biology identification process
- DNA extraction: CTAB method or commercial kit
- PCR amplification: ITS gene primers (848bp)
- Sequence alignment: The similarity with the GenBank reference sequence (MT940440.1) must be >99%
Typical experimental parameters: annealing temperature 60℃, 30 cycles, 1.5% agarose gel electrophoresis verification
Technological evolution and standard value
This standard uses the ITS sequence as the legal identification basis for the first time, which solves the limitations of traditional morphological identification in dried medicinal materials. The 21 similar species keys provided in the appendix can effectively distinguish easily confused species such as Dendrobium tassellensis and Dendrobium curcumae.
Implementation suggestions
- It is recommended to compare and identify with the morphological characteristics in Appendix B
- The DNA sample should meet the quality requirement of OD260/280 ratio of 1.7-2.0
- The standard strain with GenBank accession number MT940440.1 is recommended for positive control
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