SN/T 5669-2024 in English
VALIDDuck Parvovirus Disease Quarantine Technical Specifications
- Issued on:2024-12-31
- Implemented on:2025-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
Price(USD)：
$165.00
$161.00
$161.00
| Standard No: | SN/T 5669-2024 |
| Document status: | VALID |
| Title in English: | Duck Parvovirus Disease Quarantine Technical Specifications |
| Title in Chinese: | 鸭细小病毒病检疫技术规范 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2024-12-31 |
| Implemented on: | 2025-07-01 |
| ICS Classification: | 11.220-Veterinary medicine |
| Chinese Classification: | B41-Animal quarantine, veterinary and epidemic prevention |
| Professional Classification: | SN-Import&Export Inspection |
Introduction
Analysis of standard technical framework
| Technical modules | Core indicators | Innovation points | Application scenarios |
|---|---|---|---|
| Virus isolation | SPF duck embryo inoculation success rate ≥80% | Establishment of a standardized allantoic cavity inoculation method | Pathogen confirmation and strain preservation |
| Conventional PCR | 659bp specific band | Rep/VP1 gene dual target design | Basic laboratory testing |
| Fluorescence PCR | Ct value ≤32 was used to determine positive | VP3 gene probe quenching technology | Rapid screening and quantification |
Key technology implementation points
1. Biosafety management
Experimental operations must be carried out in a Class II biosafety cabinet, waste disposal must comply with GB19489 standards, and virus cultures must be sterilized at 121°C under high pressure for 30 minutes.
2. Sample processing specifications
- Tissue samples need to be made into a 10%-20% suspension and freeze-thawed 2-3 times to release the virus
- Cloacal swabs need to be centrifuged at 3000r/min for 10 minutes to remove impurities
- Long-term storage should be <-70℃, and repeated freezing and thawing (≤3 times) should be avoided
Comparative analysis of detection methods
Case: Epidemic diagnosis process of a certain farm
- Clinical observation: 20-day-old Cherry Valley ducks showed beak atrophy and tibia brittleness
- Spleen tissue was collected for virus isolation, and the mortality rate of duck embryos reached 60% at 96h
- Fluorescence PCR test showed a Ct value of 28.5, and sequencing confirmed that the similarity with the standard strain was 99.2%
- Comprehensively judged as DPV strong strain infection
Standard evolution and industry impact
Compared with SN/T 2123-2008, this standard has the following major upgrades:
- Newly added Real-time fluorescence PCR method, with detection sensitivity increased by 10 times
- Clarified the virus isolation verification requirements for blind passage 5
- Supplemented the gray zone result processing solution of 32≤Ct<35
Implementation suggestions
| Application scenarios | Recommended methods | Cycle | Cost control |
|---|---|---|---|
| Fast customs clearance at ports | Fluorescence PCR | 4 hours | Batch sample mixed testing |
| Breed duck farm purification | Virus isolation + sequencing | 7 days | Establish local strain library |
Sample only — not a preview of SN/T 5669-2024
Loading PDF document...
Error loading PDF. Please make sure the file is valid and try again.
We also recommend
-
SN/T 5671-2024 in English
Fluorescent PCR Detection Method for Porcine Circovirus Type 2 and Type 3
2024-12-31 -
SN/T 5335-2020 in English
Technical specifications of biosafety operations for laboratory testing African swine fever
2020-12-30 -
SN/T 4999-2017 in English
Requirements for the construction of overseas foot-and-mouth disease-free zones
2017-12-08 -
SN/T 1684-2020 in English
Technical specifications for quarantine of varroa mite disease
2020-12-30
