WS/T 369-2012 in English
VALIDDiagnosis of trichinellosis
- Issued on:2012-03-02
- Implemented on:2012-09-01
- File Format:PDF
- Delivery:Via email within 5 business days
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本标准规定了旋毛虫病的诊断依据、诊断原则、诊断和鉴别诊断。
本标准适用于各级医疗机构和疾病预防控制机构对旋毛虫病的诊断。
Introduction
Standard Overview
WS 369-2012 is a diagnostic standard for trichinellosis developed by my country's health industry, issued by the Disease Prevention and Control Bureau of the Ministry of Health and officially implemented in September 2012. The standard defines the etiological characteristics, diagnostic grading system and detection methodology requirements.
Pathogenic characteristics
| Species | Distribution area | Pathogenicity |
|---|---|---|
| Trichinella spiralis (T1) | Widely distributed throughout the country | Main pathogenic species |
| Indigenous Trichinella spiralis (T2) | Arctic/Subarctic regions | Weak pathogenicity |
Larvae are encapsulated in skeletal muscle, measuring 0.25-0.5mm×0.21-0.42mm, and usually contains 1-2 larvae.
Diagnosis grading system
1. Suspected cases
Must meet the following requirements at the same time: Epidemiological history (history of eating raw meat) + Clinical manifestations (fever/myalgia/eyelid edema, etc.)
2. Clinically diagnosed cases
Suspected cases and meet any of the following requirements:
- Larvae detected in the same batch of meat
- Eosinophils＞10%
- ELISA antibody positive
3. Confirmed cases
Clinically diagnosed cases and meet the following requirements:
- Larvae found in muscle biopsy
- Insect species identified by multiplex PCR
Laboratory testing technology
Serum testing (ELISA)
The standard recommends the use of muscle larvae ES antigen to detect IgG antibodies. The operating procedures are as follows:
- Antigen coating (2.5-10μg/mL concentration)
- Incubation with serum diluted 1:100
- HRP-labeled secondary antibody reaction
- TMB colorimetric assay
Critical value: PN ≥ 2.1 is considered positive
Muscle biopsy technique
Recommended sampling site: gastrocnemius/deltoid muscle, which can be:
- Plate microscopy (detection rate of about 30%)
- Artificial digestion (the number of larvae can be quantitatively calculated)
Implementation recommendations
Optimization of diagnostic process
It is recommended to adopt a step-by-step detection strategy:
- Initial screening: routine blood test (eosinophil count)
- Confirmation: ELISA+Western Blot combined detection
- Confirmation: muscle biopsy+molecular identification
Epidemiological monitoring
Key prevention and control areas: Southwest China (Yunnan/Sichuan/Tibet) and Northeast China, which need to be strengthened:
- Meat quarantine (especially pork/game)
- Hygiene management of holiday gatherings
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