WS/T 615-2018 in English
VALIDEstimation of radiation biological dose - analyzing premature chromosome condensation ring
- Issued on:2018-06-15
- Implemented on:2018-12-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
Price(USD)：
$160.00
$156.00
$156.00
本标准规定了通过分析早熟染色体凝集环，对电离辐射外照射受照人员进行生物剂量估算的方法。
本标准适用于发生在受照后1个月内、吸收剂量在4 Gy～20 Gy范围内的X或γ射线、急性全身均匀或近似均匀外照射的受照人员的生物剂量估算。
Introduction
Analysis of the core content of the standard
| Technical elements | Requirements of this standard | Comparison with conventional chromosome aberration methods |
|---|---|---|
| Applicable dose range | 4-20Gy | 0.25-5Gy |
| Detection time window | Within 1 month after irradiation | Optimal within 48 hours |
| Key biomarkers | PCC hollow ring | Dicentrics |
Key points of technical implementation
Key steps of specimen preparation
- After 46-47 hours of culture, calyculin A (50nmol/L) or okadaic acid (500nmol/L) was added.
- Hypotonic treatment was performed using 0.075mol/L KCl solution (37°C, 10-30min).
- The stationary phase was prepared with a ratio of methanol to glacial acetic acid of 3:1 (freshly prepared).
Establishment of dose-effect curve
The standard recommended quadratic polynomial fitting is: Y = c + αD + βD², where:
- Y: PCC ring/splitting phase
- D: Absorbed dose (Gy)
- Typical parameter example: c=0.0033, α=0.0083, β=0.00022
Quality control system
| Control link | Specific requirements |
|---|---|
| Personnel qualifications | ≥2 trained professional and technical personnel |
| Sample size requirement | Initially screen 200 split phases and calculate the total amount required according to the formula |
| Result review | All positive results require double confirmation |
Application value of the standard
This standard fills the technical gap in biological dose estimation of high-dose radiation (4-20Gy). Compared with the traditional chromosome aberration analysis method:
- The detection window period is extended to 1 month
- Differentiated interpretation standards are established for G2/M phase and M/A phase mitotic phases
- The 95% confidence interval calculation method is introduced to improve the reliability of the results
Sample only — not a preview of WS/T 615-2018
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