WS/T 775-2021 in English

VALID

Evaluation standard for disinfection effect on 2019 novel coronavirus (2019-nCoV) in laboratory

Issued on:2021-02-20
Implemented on:2021-02-20
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	WS/T 775-2021
Document status:	VALID
Title in English:	Evaluation standard for disinfection effect on 2019 novel coronavirus (2019-nCoV) in laboratory
Title in Chinese:	新型冠状病毒消毒效果实验室评价标准
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2021-02-20
Implemented on:	2021-02-20
ICS Classification:	11.080-Sterilization and disinfection
Chinese Classification:	C50-Sanitation in general
Professional Classification:	WS-Hygiene
Related Keywords:	evaluation standard disinfection effect laboratory introduction standard background standard carriers cpe effect special environment disinfection verification
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本文件规定了消毒因子对新型冠状病毒消毒效果实验室评价的基本要求、病毒灭活试验方法、结果评价和注意事项。
本文件适用于新型冠状病毒实验室消毒效果的检测与评价。

Introduction

Standard Background and Scope of Application

This standard was developed by Jiangsu Provincial Center for Disease Control and Prevention to establish a systematic laboratory testing method for the evaluation of the disinfection effect of the 2019 novel coronavirus (2019-nCoV). It is applicable to the effect verification of various disinfection factors such as ultraviolet disinfection devices and chemical disinfectants in BSL-3 laboratory environments.

Analysis of core technical requirements

Evaluation dimensions	Suspension method	Carrier method
Applicable scenarios	Evaluation of conventional disinfectants	Viscous disinfectants/ultraviolet equipment
Organic interference concentration	0.3%-3.0% bovine serum albumin	Adjust according to carrier material
Virus titer requirement	≥5.0 log₁₀	≥4.0 log₁₀
Temperature control	20±1℃	Low temperature test requires equilibration for 30min

Detailed explanation of key test methods

1. Neutralizer identification test

Four groups of control design were used to verify the effectiveness of the neutralizer: neutralizer group, neutralization product group, virus control group and cell control group. Must meet the following requirements:

The results of 3 repeated tests were consistent
The neutralization product had no inhibitory effect on the growth of Vero-E6 cells
Virus recovery rate ≥50%

2. Virus inactivation test process

Typical case:Chlorine-containing disinfectant evaluation requires the following:
① Preparation of virus suspension in a biological safety cabinet→② Disinfectant action (30s-10min)→③ Neutralizer termination reaction→④ 10-fold gradient dilution→⑤ 96-well plate inoculation→⑥ 72-hour culture to observe CPE effect

Special environment disinfection verification

For low-temperature disinfection scenarios (below 0℃), the standard specifically stipulates:

The carrier needs to be pre-cooled and balanced for ≥30min
Use stainless steel sheets as standard carriers
Conduct a 20℃ control test simultaneously

Implementation suggestions and risk control

Laboratory management requirements

Staff must have a dual background in virology and disinfection
All waste must be processed by autoclave
The experimental records should include environmental temperature and humidity monitoring data

Solutions to common problems

Problem phenomenon	Possible cause	Corrective measures
Abnormal cell control group	Contamination of culture medium	Replace with a new batch of culture medium
Virus titer does not meet the standard	Impaired cryopreservation	Use freshly passaged virus
Incomplete neutralization	Inadequate neutralizer concentration	Rerun pilot test

More samples: YD/T 3934-2021 JC/T 2453-2018 GB 50021-2001(2009)

Sample only — not a preview of WS/T 775-2021

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