GB/T 13089-2020 in English
VALIDMethod for determination of oxazolidinethione in feeds
- Issued on:2020-11-19
- Implemented on:2021-06-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$78.00
Introduction
Key points of the standard revision
| Technical parameters | 1991 edition | 2020 edition | Significance of improvement |
|---|---|---|---|
| Limit of quantification | Not specified | 150 mg/kg | Specify the method sensitivity requirements |
| Preparation of mustardase | Simple defatting | 10 times defatting + low-temperature crushing | Improve the stability of enzyme activity |
| Reaction temperature control | Not specified | 35±1.0℃ | Improve reaction reproducibility |
In-depth analysis of the principle of the method
The characteristic absorption of the cyclization product at 235-255nm was detected by UV spectrophotometer, and the matrix interference was eliminated by three-point correction method. Among them:
- Key reaction: Glucosinolate→isothiocyanate→oxazolidinethione
- Innovation: Optimize the ethanol cyclization conditions (50±1.0℃) to ensure the conversion rate>95%
Key technology for the preparation of mustard enzyme
The actual case shows that the fat content of the enzyme preparation can be reduced to <2% by using 10 petroleum ether defatting as required by the standard, and the activity retention rate can be increased by 40%. Notes during preparation:
- The crusher needs to be pre-cooled to below 10℃
- The degreasing time for each batch should be controlled at 2±0.5min
- Vacuum packaging is required when stored at -18℃
Implementation suggestions
| Steps | Operation points | Quality control indicators |
|---|---|---|
| Sample pretreatment | The oil yield of rapeseed crushing needs to be monitored at <3% | Particle size distribution detection |
| Enzymatic hydrolysis reaction | Strict timing±30s | pH7.0±0.1 |
| Centrifugal separation | Maintain 20±2℃ | Organic phase clarity |
Technical evolution analysis
Compared with the 1991 version, this standard has the following major improvements:
- Precision control: Add the requirement that the difference between parallel measurements is ≤10%
- Automation adaptation: Clarify the parameters of the vortex mixer (200 times/min)
- Environmental improvement: Reduce the amount of dichloromethane by 20%
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