GB/T 15400-2018 in English
VALIDDetermination of tryptophan in feeds
- Issued on:2018-09-17
- Implemented on:2019-04-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$156.00
《GB/T 15400-2018饲料中色氨酸的测定》由TC76（全国饲料工业标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
GB/T 15400—2018 Standard Overview
GB/T 15400—2018 is an important standard in the feed industry, used to determine the content of tryptophan in feed. This standard replaces the previous GB/T15400—1994, adds high performance liquid chromatography (HPLC), and optimizes the application scope and quantitative limit of spectrophotometry.
High Performance Liquid Chromatography (Arbitration Method)
| Project | Method Principle | Reagent Requirements | Instrument and Equipment |
|---|---|---|---|
| Principle | After tryptophan is hydrolyzed with lithium hydroxide, it is separated and quantified using an ultraviolet or fluorescence detector. | - Potassium hydroxide solution (0.1 mol/L) - Lithium hydroxide solution (4 mol/L) | - High Performance Liquid Chromatography - Ultraviolet or fluorescence detector |
| Scope of Application | Applicable to the quantitative analysis of tryptophan in feed, with a quantitative limit of 0.02%. | - Methanol (chromatographic grade) - Sodium acetate buffer solution | - Polytetrafluoroethylene hydrolysis tube - Centrifuge (≥4000 r/min) |
Method Comparison and Application
High performance liquid chromatography has high sensitivity and specificity, and is particularly suitable for trace analysis. Its chromatographic conditions require strict requirements, including mobile phase ratio and column temperature control. The spectrophotometric method is based on the characteristic reaction of tryptophan to generate a blue compound, which is suitable for rapid detection of large batches of samples.
Spectrophotometry
| Project | Reaction principle | Reagent requirements | Instruments and equipment |
|---|---|---|---|
| Principle | In sulfuric acid medium, tryptophan and p-dimethylaminobenzaldehyde condense to form a blue compound, and the absorbance is proportional to the content. | - Potassium hydroxide solution (10%) - Sulfuric acid solution (21.2 mol/L) | - Spectrophotometer - Incubator (40°C ± 2°C) |
| Scope of application | Applicable to the qualitative analysis of tryptophan in feed, with a limit of quantification of 0.04%. | - Sodium nitrite solution (0.2%) - p-Dimethylaminobenzaldehyde solution | - Centrifuge (≥4000 r/min) - Cuvette (1 cm) |
Implementation suggestions
In practical applications, it is recommended to select an appropriate method based on the fat content of the sample. For samples with low fat content, high performance liquid chromatography is preferred; for high fat samples, defatting is required first. At the same time, the laboratory should regularly calibrate the instrument and verify the accuracy of the method.
Loading PDF document...
Error loading PDF. Please make sure the file is valid and try again.
We also recommend
-
GB/T 8936-1988 in English
Bone meal and animal tankage
1988-03-30 -
GB/T 22487-2008 in English
Principle of aquafeed safety evaluation - Acute toxicity test
2008-11-04 -
GB 8830-1988 in English
Micromineral premix for layers, broilers, starter and finisher swine
-
