GB/T 15670.4-2017 in English

Interpretation of the Probability Unit Method for Acute Oral Toxicity Test

GB/T15670.4-2017 "Toxicology Test Methods for Pesticide Registration Part 4: Probability Unit Method for Acute Oral Toxicity Test" is an important standard for pesticide toxicity assessment, mainly used to determine the acute oral toxicity of pesticides. This article will provide a detailed interpretation from the aspects of test background, method key points and implementation recommendations.

I. Background of Standard Formulation

As important agricultural inputs, pesticides play a key role in preventing and controlling pests and diseases and ensuring food security. However, the toxicity of pesticides may cause harm to humans and the environment, so toxicology tests must be carried out during the pesticide registration process.

Acute oral toxicity is an important indicator for evaluating the toxicity of pesticides, and the probability unit method (Miller and Tanner method) is a classic acute toxicity assessment method. GB/T15670.4-2017 partially replaces GB/T15670—1995, and optimizes and supplements the test methods.

II. Comparison of standard frameworks

III. Interpretation of experimental methods

1. Selection and management of experimental animals

Healthy adult SD rats or Wistar rats were selected for the experiment, and the weight variation range was ±20% of the average weight of the same sex. Adaptive feeding is required for at least 3 days before the experiment, and the experiment is carried out in the environment specified in GB14925.

2. Dose design and administration method

The dose range of the formal test is determined through preliminary tests, usually with 4-5 dose groups. The exposure volume does not exceed 1mL/100g body weight. If the volume is too large, it can be given in divided doses.

3. Observation and Recording

After exposure, the poisoning symptoms and deaths of animals should be closely observed, and the reaction time and severity should be recorded. Surviving animals should be humanely killed and autopsied at the end of the experiment.