GB/T 15805.4-2018 in English

VALID

Code of diagnosis for channel catfish virus diseases

Issued on:2018-07-13
Implemented on:2019-02-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $220.00
$214.00

Standard No:	GB/T 15805.4-2018
Document status:	VALID
Title in English:	Code of diagnosis for channel catfish virus diseases
Title in Chinese:	斑点叉尾鮰病毒病诊断规程
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2018-07-13
Implemented on:	2019-02-01
Superseding:	GB/T 15805.4-2008 Quarantine methods of fish—Part 4:Channel catfish virus(CCV)
ICS Classification:	65.020.30-Animal husbandry and breeding
Chinese Classification:	B41-Animal quarantine, veterinary and epidemic prevention
Professional Classification:	GB-National Standard
Related Keywords:	catfish virus diseases introduction interpretation spotted forktail virus disease neutralization test virus dilution→antiserum treatment→cell culture observation ccv reference strain detection method old standard gb/t
Related Topics:	super virus viral disease m2 virus spot spot system shared virus tail english virus+ matting spots virus standard secretory virus diagnostic regulations virus device Epigastric virus Abnormal disease Virus Molecular Diagnostics Bit 2 single molecule virus virus security virus m2 Virus Prion disease diagnosis transient virus Device virus Pathological remote diagnosis t-virus prions female virus Prion disease ancient virus rna virus prion x4 virus virus way Foreign Pathological Diagnosis cobalt spot nor virus Catfish viral vector is a bit Virus system? Virus system? double virus disease Virus cold virus dna spot prionlow temperature component virus Bali virus broad-spectrum virus dna blob antivirus portable viral protein virus GB/T 15805.4 GB/T 15805.4-2008 GB/T 15805.4-2018 Virus pathogenicity artificial virus-like Virus single spot screening simply P virus Comparison of viral prions Viralin discontinued ev70 virus The difference between viruses, viroids, and prions Disadvantages of AAV viruses retro virus The difference between prions and viruses Kikuchi disease diagnosis virus database release

《GB/T 15805.4-2018斑点叉尾鮰病毒病诊断规程》由TC156（全国水产标准化技术委员会）归口，TC156SC11（全国水产标准化技术委员会水产养殖病害防治分会）执行，主管部门为农业农村部。

Introduction

Interpretation of the National Standard of the People's Republic of China GB/T 15805.4—2018

Standard Background and Revision Notes

GB/T 15805.4—2018 "Diagnostic Procedure for Spotted Forktail Virus Disease" replaces the old standard GB/T 15805.4—2008. The main technical changes include:

Added a detailed description of clinical symptoms
Introduced enzyme-linked immunosorbent assay (ELISA) detection method
Optimized the viral nucleic acid extraction process, and replaced the traditional method with phenol-chloroform method
Added internal control DNA for PCR detection, with a length of 186 bp
Added informative Appendix C "Spotted Forktail Virus Disease (CCVD)"

Comparison table of diagnostic methods and technical points

Detection method	Operation steps	Key reagents	Scope of application
PCR detection	DNA extraction→primer design→amplification→electrophoresis verification	Taq enzyme, dNTP, primers, internal control DNA	Confirmed cases
ELISA detection	Antigen coating→antibody binding→color reaction	Sheep anti-CCV IgG, mouse anti-CCV monoclonal antibody	Rapid screening
Neutralization test	Virus dilution→Antiserum treatment→Cell culture observation	CCV reference strain, anti-CCV antiserum	Confirm specificity

Suggestions for implementation of the standard

Case study: Fish in a farm were suspected of being infected with CCV and were diagnosed according to this standard:

Clinical symptoms: The fry swam in spasmodic movements and had a swollen abdomen
Histopathology: Enlarged kidneys and congested spleen
PCR test: The target fragment of 136 bp was amplified successfully, confirming positive

More samples: YD/T 3934-2021 JGJ 94-2008 JJG 21-2008

Sample only — not a preview of GB/T 15805.4-2018

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