GB/T 31800-2015 in English

Implementation recommendations for quarantine and identification technology of plum pox virus

Step 1: Preliminary inspection of symptoms

• Observe the symptoms of plant leaves, flowers and fruits, such as discoloration, ring spots, deformities, etc.
• Record suspicious samples and conduct subsequent laboratory tests.

Step 2: Double Antibody Enzyme-Linked Immunosorbent Assay (DAS-ELISA)

• Suitable for rapid initial screening, negative results can be directly judged as undetected.
• Positive results need to be further verified by RT-PCR.

Step 3: Molecular Biology Detection

• Conventional RT-PCR and real-time fluorescence RT-PCR are suitable for the confirmation stage and have high specificity and sensitivity.
• Real-time fluorescence RT-PCR can also perform quantitative analysis of viral load.

Step 4: Strain Identification

• Based on the test results, virus strain identification can be performed if necessary to distinguish different geographical populations and genotypes.
• Refer to the primer sequences and amplification conditions in Appendix E to ensure accuracy and repeatability.