GB/T 32757-2016 in English
VALIDMolluscs karyotype analysis
- Issued on:2016-06-14
- Implemented on:2017-01-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$78.00
《GB/T 32757-2016贝类染色体组型分析》由TC156（全国水产标准化技术委员会）归口，TC156SC2（全国水产标准化技术委员会海水养殖分会）执行，主管部门为农业农村部。
Introduction
Interpretation of GB/T 32757-2016 National Standard of the People's Republic of China "Karyotype Analysis of Shellfish"
This standard was officially implemented on January 1, 2017, aiming to standardize the method of karyotype analysis of shellfish, and is applicable to scientific research, aquaculture and biological research. This article will provide an in-depth interpretation of the background of standard formulation, technical details, instrument and equipment requirements and practical application cases.
I. Background and significance of standard formulation
| Dimensions | Content |
|---|---|
| Background analysis | With the development of aquaculture, the demand for shellfish genetic research is increasing. Chromosome karyotype analysis is the core means to reveal species evolution and genetic diversity. |
| Technological Evolution | From early microscopic observations to modern molecular biological methods, chromosome research technology has been continuously innovated. This standard integrates multiple methods (embryo method, trochophore larvae method, and adult gill tissue method) to ensure the comprehensiveness of the analysis results. |
II. Comparison of Standard Frameworks and Core Terms
1. Definition of Terms
- Heteromorphic chromosomes: Homologous chromosomes with different shapes, commonly found in shellfish.
- Secondary constriction: The area of chromosomal DNA relaxation, used to determine the nucleolar organizing region.
2. Requirements for reagents and instruments
| Category | Specific requirements |
|---|---|
| Reagents | Include analytically pure chemicals such as potassium chloride (KCl), methanol (CHOH). |
| Instruments | Such as microscope (with microphotography), centrifuge (3000×g~6000×g) and other equipment. |
III. Implementation suggestions and practical operations
1. Sample processing steps:
- Pretreatment: Select embryos, trochophore larvae or gill tissues according to different methods, and use colchicine for treatment.
- Hypotonic treatment: Use 75mol/L KCl or 25% seawater to ensure the metaphase stage of cell division.
- Fixation and dissociation: After fixation with Carnoy's fixative, dissociate with glacial acetic acid to obtain single cells.
2. Slide preparation method:
- Pressing method: Suitable for tissue block preparation, staining can be selected with reagents such as acetic acid magenta and modified carbofuxin solution.
- Drop method: Suitable for cell suspension, use Giemsa solution for staining.
3. Group type analysis:
Refer to GB/T18654.12, combine the actual samples to conduct chromosome counting and morphological observation, and pay special attention to the characteristics of heteromorphic chromosomes, secondary constrictions, etc.
Loading PDF document...
Error loading PDF. Please make sure the file is valid and try again.
