GB/T 33124-2016 in English
VALIDDetection and identification of Melampsora medusae Thümen
- Issued on:2016-10-13
- Implemented on:2017-05-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
《GB/T 33124-2016杨树叶锈病菌检疫鉴定方法》由TC271(全国植物检疫标准化技术委员会)归口,主管部门为国家标准化管理委员会。
Introduction
In-depth interpretation of the standard for quarantine identification methods for poplar leaf rust
| Dimensions | Content | Standard requirements |
|---|---|---|
| Scope of application | Pathogen detection on poplar and its host plants | Clearly stipulates the detection objects and application scenarios |
| Core method | Identification based on the morphological characteristics of summer spores | Combined with other spore organs for auxiliary identification |
| Technical equipment | Professional instruments such as biological microscopes | Clearly lists the required detection equipment |
Basic information and biological characteristics of poplar leaf rust
Chinese name:Poplar leaf rust
Scientific name:Melampsora medusa Thümen, 1878
Host range:Poplar, black cottonwood, American black poplar and pine
Transmission pathways and symptoms
Mainly spread over long distances through summer spores and winter spores. It appears as light yellow spots on the host plant, and in severe cases, the leaves dry up and fall off.
Detection and identification methods
Sample collection and preparation
- Symptom inspection: Start from the lower leaves and check for diseases layer by layer
- Sampling method: Random or targeted sampling, follow SN/T2122 standard
- Sample preparation: Including slice and needle puncture preparation techniques
Key points of morphological identification
Focus on the morphological characteristics of summer spores, such as size, shape, wall thickness and spine structure. The table compares the differences between poplar leaf rust and similar species, especially Alberta poplar and poplar leaf rust.
Implementation suggestions
1. Optimization of detection process:It is recommended to use ultra-low temperature preservation to preserve summer spores to ensure long-term sample availability.
2. Specimen management:Standardize the dry specimen production process to ensure the specimen shelf life and quality.
3. Technical training:Strengthen the microscopic operation skills and pathogen identification capabilities of testing personnel.

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