GB/T 34408-2017 in English

1. Background and significance of the standard

GB/T 34408—2017 is my country's first qualitative test method standard for cattle, sheep and pig-derived components in natural leather, filling the gap in related fields at home and abroad. The standard was proposed by the National Technical Committee for Standardization of Key Product Inspection Methods for Quality Supervision and was issued and implemented in 2017.

With the rapid development of the leather industry, mixed sources and counterfeiting and shoddy products have become increasingly prominent. This standard provides important technical support for ensuring product quality and regulating market order.

2. Comparison of standard frameworks

3. Interpretation of key technical points of the standard

3.1 Reagents and materials

The standard specifies in detail the preparation methods and quality requirements of various reagents, including:

• Analytical balance: accuracy ±0.01g
• PCR thermal cycler: used to amplify target genes
• Electrophoresis instrument: detect PCR products

All reagents must meet the corresponding quality standards to ensure the accuracy of the experiment.

3.2 Testing steps

The core process includes:

1. Decolorization: Dark leather needs to be decolorized first to avoid interference with subsequent testing.
2. DNA extraction: Purified DNA samples are obtained through multiple steps of centrifugation and washing.
3. PCR amplification: Specific primers are designed for the target species and amplified.
4. Electrophoresis: Amplification results are observed through agarose gel electrophoresis to determine whether they are positive.

4. Implementation suggestions

To ensure the effective implementation of the standard:

• Strengthen the training of testing personnel and familiarize them with PCR technology and experimental operations.
• Establish a complete quality control system and calibrate instruments and equipment regularly.
• Promote the construction of standardized testing laboratories and unify the configuration of reagents and equipment.