GB/T 34729-2017 in English

VALID

Blocking ELISA method to detect antibody against classical swine fever virus

Issued on:2017-11-01
Implemented on:2018-05-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	GB/T 34729-2017
Document status:	VALID
Title in English:	Blocking ELISA method to detect antibody against classical swine fever virus
Title in Chinese:	猪瘟病毒阻断ELISA抗体检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-11-01
Implemented on:	2018-05-01
ICS Classification:	11.220-Veterinary medicine
Chinese Classification:	B41-Animal quarantine, veterinary and epidemic prevention
Professional Classification:	GB-National Standard
Related Keywords:	classical swine fever virus blocking elisa antibody detection method classical swine fever virus introduction purified classical swine fever virus e2 protein classic swine fever virus e2 protein needs purification
Related Topics:	Elisa Antibody detection tissue elisa Viral Sequencing Methods tissue elisa tissue detection elisa GB/T34729-2017 gbt 34729 2017 gb/t34729 Antibody detection virus+ Blocking elisa detection method GB/T 34729-2017 brain elisa elisa??????? elisa+ Neutralizing antibody detection method Tissue elisa test Neutralizing antibody detection method In addition to virus detection Primary antibody detection and secondary antibody detection elisa antibody pair Detect neutralizing antibody method elisa tissue assay virus standard elisa+ tissue elisa antibody pair Antibody method Tissue elisa test Solid detection method Detection method of neutralizing antibody Absolute quantification of virus detection Neutralizing antibodies and detection antibodies Anti-virus series How to detect virus How to detect neutralizing antibody secretory virus Prion Antibody Prion Antibody New method for virus detection Food Detection Antibodies Novel virus in pigs Rapid detection of antiviral activity blocking antibody broad-spectrum antiviral elisa assay method prion pig porcine prion porcine prion prion human body Epigastric virus pig detection antibody Monoclonal antibody elisa detection Virus detection in feed virus detector Antistatic detection method Toxic gas detection method Toxic gas detection method virus test q7 organization+elisa+ ELISA detects antibodies in serum How does elisa detect antibodies elisa liquid meter Impedance system antibody hiv virus ip detection antibody elisa elisa antibody if Detection of ip antibody Anti-resistance detect ip antibody What kind of antibody elisa blocking agent antibody if How to use the antibody Antibody Upstream Detection Collagen Elisa How to use fluorescent antibody to detect virus Elisa double antibody method Which method is used to detect antibodies by elisa flow detection antibody antigen What does the elisa method generally detect? Neutralizing antibody detection method ELISA method for detection of monoclonal antibodies Epidemic virus antibody virus liquid Detection of r5 virus in vitro virus Endemic swine fever flow antibody elisa virus way if antibody Geminivirus antibody automatic elisa neutralizing antibody assay Tissue elisa test How long is the primary antibody for elisa non-viral vector Blocking Antibody Detection innate antiviral Liveness detection method Detection method of swine fever elisa secondary antibody liquid resistance viral centrosome super virus antibody double block virus human body elisa detection organization Prion detection method Prion method Anti-virus series automatic elisa elisa antibody antibody Elisa detection antibody analysis method Two strains of antibody elisa Antibodies and viruses in the body in vitro antibody blocking elisa detection antibody blocking antibody blocking antibody in vitro elisa square elisa instrument Virus sequencing detection antiviral peptide ELISA analysis gold for antibody detection GBT34729 GB/T 34729-2017 Blocking elisa and indirect elisa How is swine fever virus diagnosed blocking elisa Measuring impedance, setting impedance, action impedance difference What are the in vitro testing methods for antiviral drugs? Principle of liquid phase blocking elisa elisa HIV testing unit ELISA detection principle ELISA primary and secondary antibody species Prion detection and sampling methods Elisa has poor antigen-antibody binding ability Antibody preparation method Antiphospholipid antibody laboratory testing methods Antibody activity detection method U.S. drug testing methods swine fever place Pseudovirus purification method Antibody preparation and detection industry Determine gas toxicity Virus killing behavior detection method Impedance Spectroscopy Test Method Antibody endocytosis detection method Antiviral drug testing methods How to store primary antibodies Comparison of antibody affinity detection methods Photovoltaic ground impedance detection method Methods for preparing antibodies Methods to detect antibody titer Antibody titer detection method Diagram of syphilis detection methods Antiviral Material Testing Japan Reactor impedance detection standards Antibody synthesis methods Advantages and Disadvantages of Virus Detection Methods Antiviral drug determination methods Development of antibody activity detection methods Ketosis detection method Virus checking methods Plant virus disease detection methods New method for antibody pairing Norovirus antibody detection method Methods for detecting pseudoviruses using transmission electron microscopy Incomplete antigen detection method Virus sequencing identification and judgment Antibody internalization method Antibody targeting methods Antinuclear antibody titer detection method Neutralizing antibody titer detection method Anti-insulin antibody detection method Paired antibody detection method Anti-botulinum toxin antibody detection method Three voltmeter impedance measurement method Detection methods for anti-Golgi antibodies Square resistance detection

《GB/T 34729-2017猪瘟病毒阻断ELISA抗体检测方法》由TC181（全国动物卫生标准化技术委员会）归口，主管部门为农业农村部。

Introduction

1. Background and significance of the standard

National Standard of the People's Republic of China GB/T 34729-2017 "Blocking ELISA Antibody Detection Method for Classical Swine Fever Virus" is a standard proposed by the Ministry of Agriculture of the People's Republic of China and managed by the National Technical Committee for Animal Health Standardization. The formulation of this standard aims to provide a scientific and unified method for the detection of antibodies to classical swine fever virus and ensure the accuracy and consistency of the test results.

2. Detection Principle and Process

Blocking ELISA is a method for detecting antibodies. Its basic principle is to use antigens coated on ELISA plates to detect antibodies in samples through competitive inhibition reactions. The specific steps include:

Antigen coating: The purified classical swine fever virus E2 protein is diluted and coated on the enzyme-linked reaction plate.
Blocking treatment: Block unbound antigen sites with blocking solution.
Sample detection: Add the serum to be tested to compete with the antigen.
Enzyme conjugate reaction: Add the labeled enzyme conjugate, incubate and wash out the excess substances.
Color development and reading: Use the substrate to develop color and read the absorbance value.

3. Standard framework comparison table

Standard dimensions	Detection method	Reagent requirements	Equipment requirements	Result determination
Antigen coating	Classic swine fever virus E2 protein	Needs purification, concentration 0.2μg/mL	ELISA plate, coating solution preparation equipment	Blocking rate calculation
Enzyme conjugate	HRP-labeled monoclonal antibody	Needs to be commercialized or self-prepared, titer detection requirements	Pipette, reaction plate	OD value reading and calculation

4. Implementation recommendations

4.1 Precautions for sample collection

To ensure sample quality, it is recommended to use a disposable syringe and separate the serum as soon as possible after blood collection. It is recommended to operate under laboratory conditions to avoid environmental conditions affecting sample preservation.

4.2 Key points for reagent preparation

The expression and purification of E2 protein are key steps. It is recommended to strictly follow the requirements of Appendix A, especially the purity determination and specificity verification, to ensure the quality of the antigen. For the preparation of enzyme conjugates, it is recommended to use commercial kits. If you prepare them yourself, you need to strictly control the labeling efficiency.

4.3 Biosafety during operation

All operations should comply with laboratory biosafety regulations, especially the substrate solution and stop solution are irritating, and it is recommended to wear protective equipment. Waste disposal must comply with relevant laws and regulations.

More samples: JC/T 2453-2018 DL/T 5154-2012 QC/T 942-2021

Sample only — not a preview of GB/T 34729-2017

Loading PDF document...

Error loading PDF. Please make sure the file is valid and try again.

We also recommend

GB/T 41556-2022 in English

Diagnostic techniques for bovine Babesia infection
2022-07-11
GB/T 26618-2011 in English

Protocol of diagnosis for Perkinsosis
2011-06-16
GB/T 47130-2026 in English

Diagnostic techniques for bovine coronavirus infection
2026-01-28
GB/T 32948-2016 in English

Detection of Fecal Antigen of Echinococcus granulosus by Canine Infection by Antibody Sandwich Enzyme-linked Immunosorbent Assay
2016-08-29
GB/T 44613-2024 in English

Diagnostic techniques for bovine and caprine parainfluenza virus type 3
2024-09-29
GB/T 34720-2017 in English

Diagnostic techniques for contagious caprine pleuropneumoni
2017-11-01
GB/T 39915-2021 in English

Epidemic prevention criteria for animal breeding farm
2021-04-30
GB/T 16550-2020 in English

Diagnostic techniques for newcastle disease
2020-12-14
GB/T 36789-2018 in English

Nucleic acid detection of animal rabies virus
2018-09-17