GB/T 34748-2017 in English
VALIDGenetic diversity analysis on aquatic germplasm by genomic DNA microsatellite markers
- Issued on:2017-11-01
- Implemented on:2018-05-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
《GB/T 34748-2017水产种质资源基因组DNA的微卫星分析》由TC156（全国水产标准化技术委员会）归口，TC156SC1（全国水产标准化技术委员会淡水养殖分会）执行，主管部门为农业农村部。
Introduction
1. Background and significance of the standard
GB/T 34748—2017 is China's first national standard for genomic DNA microsatellite analysis of aquatic germplasm resources, which aims to standardize the experimental methods and data analysis processes in this field. The standard was proposed by the Ministry of Agriculture and managed by the National Technical Committee for Aquatic Products Standardization. The main drafting unit is the Heilongjiang Fisheries Research Institute of the Chinese Academy of Fishery Sciences.
2. Comparison of standard frameworks
| Standard dimensions | GB/T 34748—2017 | International similar standards | Main advantages |
|---|---|---|---|
| Scope of application | Genomic DNA microsatellite analysis of aquatic germplasm resources | Mainly for agricultural crops | Specialized in the field of aquatic products, filling the domestic gap |
| Technical requirements | Clear reagents, instruments and analysis steps | Relatively general | Detailed process, strong operability |
| Data analysis | Provide genetic parameter calculation formula | No detailed specifications | Add key indicators such as polymorphism information content |
3. Implementation suggestions and operation guidelines
3.1 Sample collection and storage
According to GB/T 18654.15, the sample size should be more than 40. The sampling location, time and method should be recorded in detail, and the "Microsatellite Analysis Record Form of Genomic DNA of Aquatic Organisms" (Appendix C) should be filled in. After the sample is collected, it should be stored immediately in accordance with the specifications to avoid degradation.
3.2 Reagent and instrument preparation
- Use Genomic DNA Extraction Kit or verify a reliable extraction method.
- Prepare microsatellite marker primers (100μmol/L) and store them properly.
- Prepare PCR amplification instruments (such as vertical electrophoresis tank and high voltage constant power electrophoresis instrument).
Loading PDF document...
Error loading PDF. Please make sure the file is valid and try again.
We also recommend
-
GB/T 20014.23-2008 in English
Good agricultural practice - Part 23: Large yellow croaker cage culture base controls points and compliance criteria
2008-02-01 -
GB/T 18654.7-2026 in English
Inspection of germplasm for fishes—Part 7:Test method of ecological characteristics
2026-02-27 -
GB/T 25877-2010 in English
Starch gel electrophoresis isozyme analysis
2011-01-10 -
GB/T 34727-2017 in English
Determination of turtles germplasm
2017-11-01 -
GB/T 8588-2024 in English
Fundamental terms of fishery resource sciences
2024-12-31 -
GB/T 40946-2021 in English
Technical guidelines for marine ranching construction
2021-11-26
