GB/T 34782-2017 in English
VALIDMethod for the determination of poplar tree gum in propolis-High performance liquid chromatography
- Issued on:2017-11-01
- Implemented on:2018-05-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
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《GB/T 34782-2017蜂胶中杨树胶的检测方法 高效液相色谱法》由TC601（全国蜂产品标准化技术委员会）归口，主管部门为中华全国供销合作总社。
Introduction
GB/T 34782—2017 Standard Overview
This standard specifies the HPLC identification method for whether propolis contains poplar gum, and is applicable to the detection of poplar gum in propolis and propolis ethanol extracts.
Background of Standard Formulation
With the rapid development of the propolis market, adulteration and counterfeiting have occurred frequently. GB/T 34782—2017 came into being, aiming to accurately identify whether propolis contains poplar gum through high performance liquid chromatography (HPLC) to ensure product quality.
Comparison of technical frameworks
| Dimensions | Standard requirements | Scope of application | Detection indicators |
|---|---|---|---|
| Detection principle | HPLC method, based on content analysis of salicin and catechol | Propolis and ethanol extracts | Salicin, catechol |
| Reagent requirements | Analytical grade reagents such as methanol, acetonitrile, ethanol, phosphoric acid, and water for first-level laboratories | - | Purity of standard products ≥98% |
| Instruments and equipment | High performance liquid chromatograph (with UV detector), grinder, rotary evaporator, etc. | - | Chromatographic column: Sepax HP-C18 |
Explanation of professional terms and practical application cases
Propolis: It is formed by the worker bees collecting resin and other plant secretions and mixing them with their maxillary gland and wax gland secretions.
Poplar Tree Gum: It comes from the buds, leaves or bark of the poplar plant, extracted with ethanol.
Case: The test results of a propolis sample showed that the content of salicin was 0.15 mg/g and the content of catechol was 0.25 mg/g, which met the standard and was judged to contain poplar gum.
Analysis of the Evolution of Standard Technology
Compared with traditional detection methods, this standard uses HPLC technology to significantly improve detection sensitivity and accuracy. The separation effect of target substances in complex matrices is ensured by optimizing the gradient elution program (see Appendix A).
Implementation Recommendations
- Sample selection:Representative propolis samples should be given priority, including raw materials and ethanol extracts.
- Operational specifications:Strictly follow the requirements for reagent preparation, instrument calibration and detection steps in the standard.
- Quality control:Perform method validation and repeatability tests regularly to ensure data reliability.
- Data interpretation:Combine the peak area integration results and the standard curve to accurately determine whether the sample contains poplar gum.
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