GB/T 34856-2017 in English

Technical requirements and method principles

This standard specifies the method for determining the content of triclocarban using high performance liquid chromatography (HPLC), and its core steps include sample preparation, solution filtration and chromatographic analysis. The following are the key points:

• Sample preparation: Weigh 0.5~1.0g of sample, extract with methanol ultrasonically and then make up the volume.
• Chromatographic conditions: C₁₈ reverse phase column, mobile phase is 70% methanol + 30% water, flow rate is 1.0mL/min, detection wavelength is 264nm.
• Quantitative method: External standard method, calculation formula is shown in formula (1). $$ X={\frac{c\times V\times1000}{m}} $$ Where, \( c \) is the sample solution concentration, \( V \) is the fixed volume, and \( m \) is the sample mass.

Comparative analysis of standards

Implementation suggestions

Laboratory operation suggestions:

• Configure reagents and calibrate instruments in strict accordance with standard requirements.
• Establish complete test records to ensure data traceability.

Manufacturers’ suggestions:

• Equip with professional testing equipment and technicians.
• Regularly send samples to third-party institutions for verification and testing.