GB/T 35330-2017 in English
VALIDDetection and identification of Hosta virus X
- Issued on:2017-12-29
- Implemented on:2018-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$243.00
《GB/T 35330-2017玉簪属植物X病毒检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
Introduction
Hostavirus X (HVX) is a disease that seriously affects ornamental plants in gardens. In order to standardize its quarantine and identification methods, the National Standard of the People's Republic of China GB/T 35330-2017 came into being. This standard combines a variety of detection technologies to ensure accurate determination of whether a sample carries the HVX virus in different scenarios.
Comparison of Standard Frameworks
| Dimensions | GB/T 35330-2017 | SN/T 2964 | SN/T 3457 |
|---|---|---|---|
| Scope of Application | Quarantine and Identification of Hosta Virus X | General Methods for Plant Virus Detection | Molecular Biological Detection of Plant Viruses |
| Core Technologies | DAS-ELISA, RT-PCR, Real-time Fluorescence RT-PCR, RT-LAMP | Virus Isolation and Identification | Genome Detection Technology |
| Update frequency | Revised regularly and adjusted according to the development of the disease | Revised every 5 years | Revised every 3 years |
Key technology analysis
DAS-ELISA test:By specifically binding the HVX virus with antibodies, rapid initial screening is achieved. This method is easy to operate and suitable for large-scale sample testing.
RT-PCR technology:Based on the genomic characteristics of HVX, specific primers are designed for amplification. This method has high sensitivity, but the reaction conditions must be strictly controlled.
Real-time fluorescence RT-PCR:Combined with fluorescently labeled probes, quantitative detection is achieved. This technology is efficient and accurate, and suitable for high-throughput sample analysis.
RT-LAMP detection:Uses loop-mediated isothermal amplification technology to complete the detection at a constant temperature. This method is particularly suitable for on-site rapid detection with limited resources.
Instruments and reagents
The following are the main instruments and reagents involved in the standard:
- Electronic balance: Sensitivity 0.001g
- PCR instrument: used for amplification reaction
- Enzyme reader: detect ELISA reaction results
- High-speed refrigerated centrifuge: separate virus particles
Implementation recommendations
To ensure the effective implementation of the standard, the following recommendations are made:
- Train quarantine personnel and familiarize them with the operating procedures of various detection technologies.
- Establish standardized laboratories and equip them with necessary instruments and equipment.
- Regularly update test kits to ensure detection sensitivity and specificity.
- Strengthen international cooperation and share HVX virus monitoring data.
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