GB/T 35332-2017 in English
VALIDDetection and identification of Grapevine virus A
- Issued on:-
- Implemented on:2018-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
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《GB/T 35332-2017葡萄A病毒检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
GB/T 35332—2017 Standard Interpretation
Standard Background and Technical Evolution Analysis
GB/T 35332—2017 "Grapevine virus A quarantine identification method" is my country's first standardized detection method for Grapevine virus A (GVA). This standard was proposed by the National Technical Committee for Plant Quarantine Standardization, and aims to standardize the quarantine of grape seedlings, scions and other propagation materials in my country to prevent the spread and spread of the virus.
With the increase in global trade, the risk of transnational transmission of plant diseases has increased, especially the GVA virus transmitted through grafting and other methods. This standard is based on internationally accepted detection methods and is formulated in combination with my country's actual situation, which has important practical significance.
Basic information of Grapevine virus A
| Classification status | Scientific name | Abbreviation | Host range |
|---|---|---|---|
| Beta-cloneviridae, Grapevine virus genus | Grapevine virus A | GVA | Mainly grape plants (Vitis spp.) |
Comparative analysis of detection methods
This standard mainly uses the following detection methods: double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), RT-PCR, real-time fluorescence RT-PCR and immunoelectron microscopy. The following table provides a detailed comparison of these methods:
| Detection method | Sensitivity | Operation complexity | Scope of application |
|---|---|---|---|
| DAS-ELISA | High | Medium | Suitable for preliminary screening |
| RT-PCR | High | High | Suitable for confirmatory detection |
| Real-time fluorescence RT-PCR | Extremely high | High | Suitable for rapid quantitative analysis |
| Immunoelectron microscopy | General | High | Mainly for research purposes |
Instrument and reagent specifications
Recommended instruments:Electronic balance, high-speed refrigerated centrifuge, PCR instrument, microplate reader, transmission electron microscope, etc.
Key reagents:DAS-ELISA reagent (Appendix B), RT-PCR detection reagent (Appendix C), real-time fluorescence RT-PCR detection reagent (Appendix D).
Implementation suggestions
Precautions in practical application:
- Ensure the validity period and storage conditions of the reagents, especially the enzymes and primers used in the PCR reaction.
- It is recommended to establish a positive sample library for regular verification of the accuracy of the detection method.
- For asymptomatic plants, RT-PCR and real-time fluorescence RT-PCR should be used for testing first.
- Cultivate samples in an isolated greenhouse to avoid the risk of virus spread.
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