GB/T 35901-2018 in English

SUPERSEDED

Real-time PCR method for detection of porcine circovirus type 2

Issued on:2018-02-06
Implemented on:2018-09-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	GB/T 35901-2018
Document status:	SUPERSEDED
Superseded by:	GB/T 21674-2025 Diagnostic techniques for porcine circovirus disease
Superseded on:	2026-03-01
Title in English:	Real-time PCR method for detection of porcine circovirus type 2
Title in Chinese:	猪圆环病毒2型荧光PCR检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2018-02-06
Implemented on:	2018-09-01
ICS Classification:	11.220-Veterinary medicine
Chinese Classification:	B41-Animal quarantine, veterinary and epidemic prevention
Professional Classification:	GB-National Standard
Related Keywords:	fluorescence pcr detection method porcine circovirus type fluorescent pcr detection application porcine circovirus type real-time pcr method
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《GB/T 35901-2018猪圆环病毒2型荧光PCR检测方法》由TC181（全国动物卫生标准化技术委员会）归口，主管部门为农业农村部。

Introduction

1. Background and significance of the standard

GB/T 35901-2018 "Fluorescence PCR Detection Method for Porcine Circovirus Type 2" is an important technical specification for the nucleic acid detection of porcine circovirus type 2 (PCV-2). The standard was proposed by the Ministry of Agriculture, managed by the National Technical Committee for Animal Health Standardization, and drafted by the Shanghai Veterinary Research Institute of the Chinese Academy of Agricultural Sciences and other units.

PCV-2 is a pathogen that seriously harms the pig industry and can cause a variety of diseases such as swine fever and reproductive failure syndrome. The fluorescence PCR detection method has become the preferred technology for diagnosing PCV-2 with its high sensitivity and specificity. The formulation of this standard provides laboratories with unified operating specifications to ensure the accuracy and reliability of the test results.

2. Comparison of standard frameworks

Standard dimensions	GB/T 35901—2018	Comparative analysis
Scope of application	Porcine circovirus type 2 nucleic acid detection	Compared with traditional methods, fluorescent PCR detection significantly improves sensitivity and specificity
Core technology	Real-time fluorescent PCR technology	Combined with internal reference plasmids (IC-F, IC-R) and probes (PCV2-P, IC-P) to ensure the accuracy of the amplification curve
Detection limit	Ct value ≤ 38 is reported as positive, Ct value between 38-40 is suspicious	Repeat the experiment to ensure the reliability of the result

Table 1: Comparison of GB/T 35901-2018 standard framework

3. Implementation suggestions and precautions

3.1 Laboratory conditions

Equipped with key equipment such as fluorescent PCR detector and high-speed refrigerated centrifuge
The laboratory environment must meet the biosafety level 2 (BSL-2) standard
Reagents and consumables must be purchased and stored in strict accordance with the standard requirements

3.2 Sample collection and processing

According to the standard requirements, sample collection includes:

Serum sample: 5 mL, centrifuge to obtain supernatant
Feces sample: Take 1 g sample and add PBS buffer to mix
Tissue sample: Take 1 g tissue homogenate and centrifuge at 8000r/min for 5 minutes

3.3 Suggestions for Optimization of Operation Procedure

In fluorescence PCR operation:

DNA extraction: Use DNAzol or commercial kit to ensure DNA purity
Reaction system preparation: Strictly prepare according to the sample loading volume in Table C.1
On-machine detection: The fluorescence channels are set to FAM and HEX, and the cycling conditions need to be strictly controlled

4. Conclusion

GB/T The implementation of the 35901-2018 standard is of great significance to improving the disease prevention and control level of my country's pig farming industry. Laboratories should strictly operate in accordance with the requirements of this standard to ensure the accuracy of test results, and regularly participate in proficiency testing to improve the overall testing level.

More samples: DL/T 368-2010 JJG 21-2008 JGJ 94-2008

Sample only — not a preview of GB/T 35901-2018

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