GB/T 35939-2018 in English
VALIDIndirect enzyme linked immunosorbent assay for detecting the antibody against encephalomyocarditis virus
- Issued on:2018-02-06
- Implemented on:2018-09-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
《GB/T 35939-2018脑心肌炎病毒间接ELISA抗体检测方法》由TC181（全国动物卫生标准化技术委员会）归口，主管部门为农业农村部。
Introduction
1. Background and significance of standard formulation
Encephalomyocarditis (ECM) is an infectious disease caused by encephalomyocarditis virus (EMCV) and is widely found in pigs and rodents. The disease can cause clinical symptoms such as acute fatal myocarditis in piglets, reproductive disorders in sows and encephalitis in humans, posing a serious threat to animal husbandry and public health safety.
The formulation of this standard aims to standardize the detection method of encephalomyocarditis virus antibodies and provide a scientific basis for disease prevention and control. The standard was drafted by Nanjing Agricultural University and China Animal Health and Epidemiology Center and was officially released in 2018.
2. Comparison table of standard frameworks
| Standard dimensions | GB/T 35939—2018 | Internationally accepted standards | Difference analysis |
|---|---|---|---|
| Detection method | Indirect ELISA method | RT-PCR and immunofluorescence method are the main methods | Domestic standards focus more on antibody detection, while international standards mostly use a combination of nucleic acid and antigen detection |
| Scope of application | Porcine encephalomyocarditis virus antibody detection | Widely used in the detection of various animal pathogens | Domestic standards focus on specific diseases, while international standards are more universal |
| Requirements for reagents | Standardized antigens and control serum are required | Supports a variety of commercial kits | Domestic standards have detailed specifications for reagent preparation, and the international market relies more on commercial products |
3. Recommendations for standard implementation
Laboratory preparation:
- Equipped with instruments and equipment such as microplate readers, centrifuges and pipettes required by the standard.
- Establish a standardized reagent preparation process, especially for antigen coating solutions and substrate solutions.
Sample collection and processing:
- Collect pig blood samples in accordance with NY/T 541.
- Strictly perform serum separation operations to ensure that the samples are free of hemolysis and contamination.
Detection process optimization:
- It is recommended to use an automated pipetting system to improve work efficiency.
- Establish a positive and negative control quality control system to ensure detection accuracy.
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