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disinfection efficacy test method atp bioluminescence method introduction interpretation food contact surfaces cleaning food contact surfaces prospect atp bioluminescence method atp bioluminescence standard quality assurance proceduresimplementation machinery safety control systems part tomography scopethis standard
GB/T 36004-2018 in English

GB/T 36004-2018 in English

VALID

Food contact surfaces cleaning and disinfection efficacy test method—ATP bioluminescence method

  • Issued on:2018-03-15
  • Implemented on:2018-10-01
  • File Format:PDF
  • Delivery:Via email within 1~3 business days
Price(USD): $80.00
$78.00
Standard No: GB/T 36004-2018
Document status: VALID
Title in English: Food contact surfaces cleaning and disinfection efficacy test method—ATP bioluminescence method
Title in Chinese: 食品接触表面清洗消毒效果试验方法 三磷酸腺苷生物发光法
Language: English
File Format: Electronic (PDF)
Delivery: Via email within 1~3 business days
Issued on: 2018-03-15
Implemented on: 2018-10-01
ICS Classification: 71.100.40-Surface active agents
Chinese Classification: Y43-Tooth paste, soap and detergent
Professional Classification: GB-National Standard
Related Keywords: disinfection efficacy test method atp bioluminescence method introduction interpretation
food contact surfaces cleaning
food contact surfaces
prospect atp bioluminescence method
atp bioluminescence
Related Topics: surface microbial contact plate
Adenosine in Cordyceps
Contact sheet cleaning
GB/T 36004-2018
Lighting principle
Bioluminescence
Zirconium Phosphate Food Contact
Bioluminescence
Digestive acid
triple acid digestion
Luminescence
superficial items
food contact
GBT36004
GB/T 36004-2018
Microbiological standards for food contact surfaces
Cytosine nucleoside triphosphate test method
Test method for effectiveness of cleaning and disinfection of food contact surfaces
Adenosine detection method
Adenosine monophosphate detection method
Food contact surface microorganisms
Adenosine deaminase assay method
Sanpu available phosphorus test method
Preparation method of adenosine
Surface Microbial Contact Disk Detection
Adenosine Pharmacopeial Methods
Mecon Bioluminescence Methodology
Adenosine detection method
Cyclic adenosine monophosphate element test method

《GB/T 36004-2018食品接触表面清洗消毒效果试验方法 三磷酸腺苷生物发光法》由TC395(全国食品用洗涤消毒产品标准化技术委员会)归口,主管部门为中国轻工业联合会。


Introduction

Interpretation of GB/T 36004—2018, the National Standard of the People's Republic of China


Analysis of the Background of Standard Formulation and Technology Evolution

GB/T 36004—2018 is based on the principle of adenosine triphosphate (ATP) bioluminescence and is used to evaluate the cleaning and disinfection effects of food contact surfaces. This method quantifies the amount of biological residues by detecting the relative luminescence unit (RLU) of ATP.

Compared with traditional microbial detection, ATP bioluminescence is fast, sensitive and does not require cultivation. This technology has been widely used in the food industry, especially for high-precision disinfection effect evaluation.


Comparison of two detection methods

Method Applicable scenarios Operation steps Precautions
Handheld ATP bioluminescence detector smear method Rapid on-site detection, suitable for daily monitoring of food processing enterprises.
  • Smear the surface of the sampling stick and activate the reaction
  • Insert the instrument to detect the RLU value
a) Avoid touching the sampling head with your hands; b) Unify the smearing method to ensure repeatability.
Desktop chemiluminescence analyzer detection method Precise laboratory analysis, suitable for scientific research and quality control.
  • Use filter paper to collect samples and lyse cells
  • Extract supernatant for testing after centrifugation
a) Use clean glassware; b) Avoid repeated freezing and thawing of luciferase.

Recommendations for implementation of standards

Practical application case analysis

A food company uses a handheld ATP detector to conduct daily monitoring of the equipment surface in the production workshop. By comparing the RLU values before and after cleaning and disinfection, it was found that the amount of biological residues was reduced by more than 85%.

Suggestions:

  • Enterprises should adjust cleaning and disinfection procedures based on test results
  • Choose appropriate ATP detection methods (handheld or desktop)
  • Establish a long-term monitoring mechanism for cleaning and disinfection effects

Conclusion and Prospect

ATP bioluminescence method is an important tool for evaluating the cleaning and disinfection effects of food contact surfaces. It is fast, sensitive and objective. With the advancement of technology, this method will become more intelligent and automated in the future, providing stronger protection for food safety.

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