GB/T 36194-2018 in English

VALID

Detection method of goldfish haematopoietic necrosis virus

Issued on:2018-05-14
Implemented on:2018-12-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	GB/T 36194-2018
Document status:	VALID
Title in English:	Detection method of goldfish haematopoietic necrosis virus
Title in Chinese:	金鱼造血器官坏死病毒检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2018-05-14
Implemented on:	2018-12-01
ICS Classification:	65.020.30-Animal husbandry and breeding
Chinese Classification:	B41-Animal quarantine, veterinary and epidemic prevention
Professional Classification:	GB-National Standard
Related Topics:	necrosis Viral Sequencing Methods Necrosis detection Hemolytic toxin detection hematopoietic necrosis virus necrotic baculovirus Hemolytic toxin detection fish hematopoietic cell virus Epidemic hematopoietic necrosis disease quarantine virus detection blood virus In addition to virus detection How to detect virus New method for virus detection Necrosis detected by flow cytometry streaming necrosis virus detector test organ blood detector Virus detection method fish serum Hematopoietic virus detection lots of dead fish Necrosis detection method Cardiovascular Disease Detection Methods goldfish hematopoietic virus GB/T36194-2018 Detection method of hematopoietic necrosis virus in goldfish gold for pathogen detection gb/t36194 Detection of r5 virus Plasma detection method virus way Necrosis detection How is necrosis detected Prion detection method Prion method Virus sequencing detection GBT36194 GB/T 36194-2018 Blood fluoride test blood gold test How to use blood lipid detector Blood lead detection method Which test is used to detect serum IL8? Toxicity of ECD detector Prion detection and sampling methods blood drug test Virus detection methods Blood heavy metal detection method Blood culture testing methods Advantages and Disadvantages of Serological Testing Methods Pseudovirus purification method Reference electrode quality detection method Blood copper ion detection method Endothelin detection method Virus killing behavior detection method Drug testing fee How to kill chickens Lipid meter detection method Antiviral drug testing methods What are the instrument testing methods for syphilis? Blood type test fee Cell necrosis detection gold Blood metal ion detection method Syphilis blood station detection method Ketosis detection method Virus checking methods Blood parenteral toxin detection method Drug testing fee Eight detection methods before blood transfusion Organoid quantification methods The correct way to draw blood and disinfect it Quantitative detection methods for organoids Four detection methods for new blood clots Erythrocyte sedimentation rate detection method Extravascular hemolysis detection method

《GB/T 36194-2018金鱼造血器官坏死病毒检测方法》由TC156（全国水产标准化技术委员会）归口，TC156SC11（全国水产标准化技术委员会水产养殖病害防治分会）执行，主管部门为农业农村部。

Introduction

1. Standard Overview

National Standard of the People's Republic of China GB/T 36194-2018 specifies the detection method of goldfish hematopoietic necrosis virus (cyprinid herpesvirus type 2), including PCR and fluorescent PCR technology.

2. Detailed Interpretation of the Detection Method

Comparison Dimensions	PCR Detection	Fluorescent PCR Detection
Reagent Requirements	Requires dNTP, Taq DNA polymerase, primers, etc.	Requires fluorescent PCR premix, probe, etc.
Test steps	1. DNA extraction; 2. PCR amplification; 3. Electrophoresis detection.	1. DNA extraction; 2. Reaction system preparation; 3. Fluorescence PCR amplification.
Result determination	Determine based on specific bands and sequencing results.	Determine based on Ct value and amplification curve.

3. Implementation suggestions

In actual operation, it is recommended that:

Ensure that the laboratory has the corresponding biosafety level.
Staff must receive professional training.
Calibrate equipment regularly to ensure accuracy.
Establish a quality control system.

More samples: GB 50021-2001(2009) NB/T 47013.2-2015 GB 18306-2015

Sample only — not a preview of GB/T 36194-2018

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