GB/T 36752-2018 in English
VALIDDetection and identification of Sowbane mosaic virus
- Issued on:2018-09-17
- Implemented on:2019-04-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
《GB/T 36752-2018藜草花叶病毒检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
1. Background and significance of standard formulation
GB/T 36752-2018 "Quarantine and identification methods for Chenopodium album mosaic virus" is a domestic standard formulated according to international plant quarantine standards, aiming to standardize the detection process of Chenopodium album mosaic virus (Sowbane mosaic virus, SoMV). The virus is mainly transmitted mechanically and through seeds, and harms a variety of economic crops and fruit trees.
2. Comparison of standard frameworks
| Standard dimensions | GB/T 36752—2018 | Differences in international standards |
|---|---|---|
| Detection method | DAS-ELISA, RT-PCR, real-time fluorescence RT-PCR | In line with international standards, using advanced molecular biology technology |
| Scope of application | Plant seeds, seedlings and products | Covering major economic crops and fruit trees |
| Detection sensitivity | Can detect trace amounts of viruses | Reaching the international advanced level |
3. Detection principle and technical path
Detection principle: Based on the serological characteristics and genomic characteristics of the virus, multiple methods are used for joint detection. Specifically including:
- DAS-ELISA: Double antibody sandwich enzyme-linked immunosorbent assay
- RT-PCR: Reverse transcription polymerase chain reaction
- Real-time fluorescence RT-PCR: Real-time monitoring of virus amplification
4. Implementation suggestions
4.1 Preparation and preservation of test samples
Seed samples should be selected from deformed seeds or plants showing symptoms, and seedlings and plant products should be sampled in accordance with SN/T 2122. Test samples need to be stored at different temperatures: 4℃ for seeds, -80℃ for seedlings and leaves, for review.
4.2 Selection of detection methods
Choose an appropriate combination of methods according to the detection requirements. For suspected samples, it is recommended to perform DAS-ELISA and RT-PCR tests at the same time to improve accuracy.
4.3 Recording and reporting of results
Record the sample source, detection time, method and results in detail. Enzyme-linked reaction values, electrophoresis images and fluorescence curves should be saved as important attachments.
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