GB/T 36770-2018 in English
VALIDDetection and identification of Australian grapevine viroid
- Issued on:2018-09-17
- Implemented on:2019-04-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
《GB/T 36770-2018澳洲葡萄类病毒检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
National Standard of the People's Republic of China GB/T 36770—2018: In-depth Interpretation
GB/T 36770—2018 is a quarantine and identification method standard for Australian grapevine viroid (AGVd). The standard provides detailed testing procedures, reagent formulations and equipment requirements to ensure the accuracy and consistency of test results.
I. Comparison of Standard Frameworks
| Standard Dimensions | GB/T 36770—2018 | International Reference Standard |
|---|---|---|
| Scope of Application | Applicable to the quarantine and identification of grape propagation materials. | In line with the International Agreement on Phytosanitary Measures (IPPC). |
| Detection methods | RT-PCR, real-time fluorescence RT-PCR and dot hybridization. | Molecular biology techniques are recommended. |
| Reagent requirements | The reagent purity and preparation methods are specified in detail. | In line with international general reagent standards. |
II. Technical interpretation
1. Detection principle
The detection of Australian grape virus is mainly based on its nucleic acid sequence. Through reverse transcription polymerase chain reaction (RT-PCR) and nucleic acid molecular hybridization methods, the RNA fragments of the virus can be specifically amplified or identified.
2. Reagents and instruments
- Main reagents:Including buffers and primers required for ordinary RT-PCR and real-time fluorescence RT-PCR, all of which must be analytical grade or above.
- Key instruments:High-speed refrigerated centrifuge (for nucleic acid extraction), PCR instrument (amplification of DNA fragments) and real-time fluorescence quantitative PCR instrument (for quantitative detection).
III. Implementation suggestions
1. Laboratory construction
It is recommended to establish a laboratory that meets the biosafety level 2 (BSL-2) standard, equipped with special equipment and ventilation system to ensure the safety of the detection process.
2. Operation specifications
- Strictly abide by the aseptic operation procedures to avoid contamination.
- Regularly calibrate the instruments and equipment to ensure the accuracy of the test results.
- Strengthen staff training to ensure that they master the latest testing technology.
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