GB/T 36780-2018 in English
VALIDDetection and identification of Pepper mild mottle virus
- Issued on:2018-09-17
- Implemented on:2019-04-01
- File Format:PDF
- Delivery:Via email within 5 business days
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《GB/T 36780-2018辣椒轻斑驳病毒检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
National Standard of the People's Republic of China GB/T 36780—2018 Quarantine and Identification Methods for Pepper Mild Mottle Virus
1. Scope and Application of the Standard
This standard specifies the quarantine and identification methods for pepper mild mottle virus (PMMoV), which is applicable to virus detection on host plants and untreated seeds. This standard provides important technical support for pepper cultivation, international trade and plant quarantine.
2. Basic information of the virus
| Item | Content |
|---|---|
| Scientific name | Pepper mild mottle virus (PMMoV) |
| Chinese name | Pepper mild mottle virus |
| Classification status | Virgaviridae, Tobamovirus |
| Host range | It mainly parasitizes plants of the genus Capsicum, and can also infect plants of the Solanaceae family such as tomato and tobacco. |
3. Detection Principles and Methods
The detection of PMMoV is based on its protein and genome characteristics, using a variety of immunological and molecular biological techniques:
- DAS-ELISA: Double antibody sandwich enzyme-linked immunosorbent assay.
- Dot-ELISA: Dot enzyme-linked immunosorbent assay.
- Colloidal gold immunochromatography assay: Rapid detection of viruses in diseased leaves.
- RT-PCR: Reverse transcription polymerase chain reaction.
- Real-time fluorescence RT-PCR: Quantitative detection of viral nucleic acid.
4. Testing Process and Quality Control
Case Study: Seed Sampling and Testing
To ensure the detection rate of seed-borne diseases, according to Appendix G, the seed sampling volume needs to be determined based on the infection level. For example, when the infection level is 0.1%, at least 3,000 seeds should be sampled, with a maximum of 250 seeds per sample. The test results should be determined by combining double verification of ELISA and RT-PCR/real-time fluorescence RT-PCR.
5. Implementation Recommendations
- Application of the Standard: This standard should be used preferentially in pepper cultivation, international trade and plant quarantine.
- Reagent Selection: Use highly sensitive DAS-ELISA kits and validated commercial one-step RT-PCR/real-time fluorescence RT-PCR kits.
- Quality Control: Strictly control sample extraction, reagent preparation and testing environment to ensure the accuracy of results.
- Staff Training: Provide standardized operation training for quarantine technicians to improve testing capabilities.
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