GB/T 36820-2018 in English

VALID

Detection method of Sugarcane streak mosaic virus by real-time reverse transcription polymerase chain reaction（RT-PCR）

Issued on:2018-09-17
Implemented on:2019-04-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	GB/T 36820-2018
Document status:	VALID
Title in English:	Detection method of Sugarcane streak mosaic virus by real-time reverse transcription polymerase chain reaction（RT-PCR）
Title in Chinese:	甘蔗条纹花叶病毒实时荧光反转录聚合酶链式反应（RT-PCR）检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2018-09-17
Implemented on:	2019-04-01
ICS Classification:	65.020.01-Farming and forestry in general
Chinese Classification:	B16-Plant quarantine, pest control
Professional Classification:	GB-National Standard
Related Topics:	pcr polymerase series flow fluorescent pcr Multiplex fluorescent PCR enzyme real-time polymerase chain reaction react-time test Reverse transcription and detection Anti-t3 detection method reverse transcription chain RT-strand transcription method fluorescence response fluorescent signal isothermal pcr enzyme Fluorescence detection method by flow cytometry How to detect after reverse transcription How to detect reverse transcription Anti detection abnormal pcr streaks pcr reaction 4 degrees ELISA Enzyme cup pcr real time polymerase chain reaction rt-pcr reaction instrument fluorescence derivatization reaction real-time polymerase chain reaction retrovirus Real-time fluorescence quantitative polymerase chain reaction instrument enzyme reactor pcr polymerase chain reaction rt reaction real-time quantitative polymerase chain reaction rt reaction reverse pcr pcr+ polymerase carbon chain reaction fluorescence response several pcr polymerases pcr reaction enzyme when rt reacts standard pcr reaction flow real-time fluorescence reverse polymerase chain reaction light reaction two pcr luciferase Enzyme zero order reaction Fluorescent reaction detection T4 polymerase in pcr pcr enzyme reverse polymerase chain reaction Reverse transcription and polymerase chain reaction pcr amplification reaction pcr reaction if reverse polymerase chain reaction organic reaction rt How to detect after reverse transcription Detection by RT-PCR pcr flow cytometry fluorescence detection pcr technology polymerase Secondary reactions of enzymatic reactions enzymatic reaction secondary reaction When is the transcript single-stranded Enzyme-catalyzed reaction double enzyme reaction pcr amplification reaction Quantitative fluorescent polymerase chain reaction pcr reaction box pararetrovirus pcr reaction? enzyme time pcr instrument Enzyme fluorescence detection non-spontaneous enzyme rt reaction and pcr reaction GBT36820 GB/T 36820-2018 Photosynthetic dark reaction parameters pcr reaction program settings Reverse transcription polymerase reaction to detect liver glyceraldehyde test nitrification reaction, fluorescence Nitration reaction, fluorescence reaction principle photosynthetic reaction carbon output association reaction catase reaction Method for measuring dual luciferase using a fluorescent microplate reader Methods to detect enzymatic reactions Methods for experimentally measuring reaction series Luciferase Assay Method Luciferase luminescence detection method using microplate reader Methods for detecting reaction membranes using biochemical test strips Sugarcane leaf sampling method Polymerase chain reaction method Luciferase detection method Exploration Methods Fluorescence Reaction Fluorescence Reaction Fluorescence Reaction Identification Method

《GB/T 36820-2018甘蔗条纹花叶病毒实时荧光反转录聚合酶链式反应（RT-PCR）检测方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

Real-time fluorescence reverse transcription polymerase chain reaction detection method for sugarcane streak mosaic virus

1. Background and significance of standard formulation

Sugarcane streak mosaic virus (SCSMV) is a quarantine disease that seriously affects sugarcane production. The formulation of this standard aims to provide a scientific basis for the rapid and accurate detection of SCSMV in sugarcane and ensure the healthy development of the sugarcane industry. This standard is proposed and managed by the National Technical Committee for Plant Quarantine Standardization, and the main drafting units include the National Sugarcane Engineering Technology Research Center of Fujian Agriculture and Forestry University.

2. Principle of the method

Based on the CP gene sequence of the SCSMV virus, specific primers and probes are designed, and the viral RNA is converted into cDNA using reverse transcriptase, and real-time fluorescence PCR amplification is performed in the same reaction tube. The presence of the target sequence is monitored by the change in the fluorescence signal of the TaqMan probe to achieve rapid detection.

3. Comparative analysis of standard frameworks

Standard dimensions	GB/T 36820—2018	Comparison description
Detection principle	Real-time fluorescence RT-PCR combined with TaqMan probe technology	Compared with the traditional ELISA method, the sensitivity and specificity are significantly improved
Detection range	Suitable for rapid detection of living host plants of sugarcane	Specially designed for sugarcane quarantine, highly targeted
Instrument requirements	Real-time fluorescence PCR instrument (excitation/detection wavelength range: 350nm~750nm)	Strict equipment performance indicators to ensure data accuracy

4. Implementation suggestions

Precautions in practical application

Sample collection and pretreatment:Ensure the sterilization of tools to avoid cross contamination. Leaf and stem samples need to be processed separately and stored at 2~8℃ for a short period of time or -80℃ for a long period of time.

Reagent preparation:Strictly follow the principle of nuclease-free water preparation. PCR reaction solution needs to be prepared before use to avoid repeated freezing and thawing that affects the effect.

Data analysis:Ct value is the key indicator (positive: Ct≤35; negative: Ct≥40), which needs to be combined with the amplification curve for comprehensive judgment.

More samples: JGJ 94-2008 YD/T 3934-2021 QC/T 942-2021

Sample only — not a preview of GB/T 36820-2018

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