GB/T 36831-2018 in English
VALIDDetection and identification of Cronartium fusiforme Hedgcock & Hunt ex Cummins
- Issued on:2018-09-17
- Implemented on:2019-04-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
| Standard No: | GB/T 36831-2018 |
| Document status: | VALID |
| Title in English: | Detection and identification of Cronartium fusiforme Hedgcock & Hunt ex Cummins |
| Title in Chinese: | 松纺锤瘤锈病菌检疫鉴定方法 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2018-09-17 |
| Implemented on: | 2019-04-01 |
| ICS Classification: | 65.020.01-Farming and forestry in general |
| Chinese Classification: | B16-Plant quarantine, pest control |
| Professional Classification: | GB-National Standard |
| Related Topics: | superbugs
tumor generation tumor affairs Identification of flora tumor mass Spindle wheel GBT36831 GB/T 36831-2018 Complex bacteria identification algorithm Purification methods of rhizobia Stripe rust inoculation method Casson detection method Quarantine and identification method of chrysanthemi rot fungus Methods for Identifying Plant Fungal Pathogens |
《GB/T 36831-2018松纺锤瘤锈病菌检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
Interpretation of GB/T 36831—2018 "Quarantine and Identification Methods for Pine Spindle Tuber Rust"
Background and Significance of Standard Formulation
GB/T 36831—2018 "Quarantine and Identification Methods for Pine Spindle Tuber Rust" is to standardize the quarantine and identification of pine spindle tuber rust (Cronartium fusiforme) to ensure the safety and compliance of host plants and their products in international trade. The standard was proposed and managed by the National Technical Committee for Plant Quarantine and Standardization, and the main drafting units include entry-exit inspection and quarantine bureaus and scientific research institutions in Shenzhen, Shandong, Zhejiang and other places.
Basic information of pathogens
Cronartium fusiforme belongs to the phylum Basidiomycetes and the subphylum Puccinidium, and mainly parasitizes pine plants and alternate hosts such as Quercus. Its main transmission route is air flow, and similar species include Pinus tabulaeformis and Pinus blister rust.
Overview of identification methods
| Identification steps | Operation points | Key instruments |
|---|---|---|
| Symptom inspection | Observe whether the host plant has tumors, nectar droplets overflowing, and spore organs. | microscope, stereomicroscope, etc. |
| Morphological identification | Identification is carried out by observing the morphological characteristics of rust spores, summer spores and winter spores. | PCR amplification instrument, electrophoresis instrument, etc. |
| Nucleic acid sequence alignment | PCR amplification is performed using ITS1/ITS4 primers, and the sequence is aligned with the GenBank database. | Autoclave, Constant temperature water bath, etc. |
Comparison of morphological characteristics
The morphological characteristics of pine spindle tumor rust fungus and its similar species are compared as follows:
| Pathogen | Symptoms | Rust spores | Sudden spores | Tylospores |
|---|---|---|---|---|
| Cronartium fusiforme | Tumors appear on the trunk and branches, which burst to expose the wood. | Spherical to fusiform, with thin walls and wart-like protrusions. | Oval to elliptical, with uniform and sharp small spines on the surface. | Cylindrical to fusiform, smooth wall, size (35μm~70μm)×(14μm~27μm). |
| Cronartium comandrae | Orange-red rust spores appear on the host, and yellowish spots appear on the leaves of the host. | Long ovate or obovate, with wart-like protrusions on the wall. | Oval or obovate, slightly yellowish and transparent, with thorns on the surface. | Short elliptical, transparent wall, size (25μm~75μm)×(9μm~18μm). |
Laboratory Records and Preservation
According to the standard requirements, the laboratory needs to properly preserve the test report, samples, nucleic acid and other information. The test report should indicate the date, method and results, and be signed by the experimenter and the auditor.
Implementation recommendations
- Strengthen port quarantine efforts, focusing on host plants and their products from pine spindle rust epidemic areas.
- The laboratory should be equipped with necessary instruments and equipment (such as PCR instrument, electrophoresis instrument, etc.) to meet testing needs.
- Carry out regular technical personnel training to improve the ability to identify pine spindle rust and its similar species.
Summary
GB/T 36831—2018 "Quarantine and Identification Methods for Pine Spindle Rust" provides a scientific basis and operational guidelines for standardizing the detection of the pathogen, which is of great significance for protecting the safety of my country's forest resources. By strictly implementing this standard, the spread of pine spindle rust can be effectively prevented.
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