GB/T 36833-2018 in English

National Standard of the People's Republic of China GB/T 36833—2018

Potato virus X quarantine identification method

Background and significance of standard formulation

The formulation of GB/T 36833—2018 is based on the important position of potato in international trade and agricultural production. Potato virus X (PVX) is a quarantine disease that seriously affects potato yield and quality. The introduction of this standard fills the gap in the field of PVX detection methods in China, and provides a scientific basis for standardizing import and export plant quarantine and ensuring agricultural safety.

Detection principle and technical route

The genomic characteristics and immunogenicity of PVX are the main basis for its quarantine identification. The standard adopts a variety of molecular biology techniques and immunological methods, including:

• Reverse transcription PCR (RT-PCR)
• Real-time fluorescence reverse transcription PCR (Real-time RT-PCR)
• Reverse transcription loop-mediated isothermal amplification (RT-LAMP)
• Enzyme-linked immunosorbent assay (ELISA)
• Immunochromatographic test strip test
• Dot enzyme-linked immunosorbent assay (dot-ELISA)

Main instruments and equipment and function comparison table

Sample preparation and sampling methods

The standard specifies a detailed sample preparation process, including:

1. Cultivation and observation of tissue culture seedlings, miniature potatoes, and tubers
2. Sowing and symptom monitoring of host plant seeds of the Solanaceae family
3. Direct detection of samples with suspicious symptoms

Result determination and record keeping

The detection result is determined by a double confirmation mechanism:

• Negative samples must be negative by at least one method
• Positive samples must be positive by at least two methods

Implementation suggestions and precautions

In order to ensure the effective implementation of the standard, the following suggestions are put forward:

1. Laboratory conditions:Equipped with Class II biosafety protection facilities.
2. Staff training:Regularly conduct training on testing methods and quality control.
3. Reagent management:Establish a reagent validity period and traceability record system.
4. Data sharing:Promote the information management and sharing mechanism of quarantine results.