GB/T 36843-2018 in English

1. Background and significance of the standard

GB/T 36843-2018 "Pear decline phytoplasma quarantine and identification method" is an important standard for pear decline disease in China, aiming to standardize the quarantine and identification process and prevent the spread of the disease. The standard was proposed by the National Technical Committee for Plant Quarantine Standardization and approved by the National Standardization Administration.

2. Basic information of pear decline phytoplasma

3. Detection methods and technical points

This standard mainly adopts molecular biological detection technology, including:

• Nested PCR amplification and detection: Use universal primers P1/P7 and specific primers fPD/rPDs for amplification.
• Electronic restriction endonuclease pattern (RFLP) analysis: Establish a fingerprint map through sequencing results and compare it with the standard map.
• Real-time fluorescence PCR detection: Specific amplification of 16S rDNA sequence.

4. Implementation suggestions